Expanding the applicability of Baeyer-Villiger Monooxygenases

Research output: ThesisThesis fully internal (DIV)

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Abstract

In the thesis “Expanding the applicability of Baeyer-Villiger monooxygenases” the use of this class of enzymes is facilitated in different areas. Enzymes are increasingly used in to replace purely chemical processes. By stabilizing enzymes, changing enzymes so they are able to catalyze the required reactions and by simplifying the use of enzymes in different ways a next step towards industrial applications is made. The potential applications of Baeyer-Villiger monooxygenases range from high-value pharmaceutical compounds such as prazoles and modafinil to bulk chemicals that are for example used for the production of Plexiglas. For each of these applications patents have been granted.
The increasing applicability of these enzymes is achieved via different routes. Two different enzymes with different desired properties have been combined to create a hybrid-enzyme that inherited the desired properties of both enzymes. The expanding possibilities of computational design have been used to introduce new properties. Based on a model of the enzyme stabilizing changed have been calculated, that were subsequently applied to the enzyme successfully.
In the realm of the bio-based economy a NWO financed TASC project was started to produce a polymer-precursor (for Plexiglas) based on renewable feedstock. Several Baeyer-Villiger monooxygenases were found to catalyze the last step in this process.
In summary this lead to improvements that facilitate the use of Baeyer-Villiger monooxygenase. In addition, methods to achieve these improvements have been developed.
Translated title of the contributionHet vergroten van de toepasbaarheid van Baeyer-Villiger Monooxygenases
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
Supervisors/Advisors
  • Fraaije, Marco, Supervisor
Award date31-Oct-2014
Place of Publication[S.l.]
Publisher
Print ISBNs978-90-367-7330-0
Electronic ISBNs978-90-367-7331-7
Publication statusPublished - 2014

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