Expanding the enzyme universe with genetically encoded unnatural amino acids

Ivana Drienovska*, Gerard Roelfes*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)
48 Downloads (Pure)

Abstract

The emergence of robust methods to expand the genetic code allows incorporation of non-canonical amino acids into the polypeptide chain of proteins, thus making it possible to introduce unnatural chemical functionalities in enzymes. In this Perspective, we show how this powerful methodology is used to create enzymes with improved and novel, even new-to-nature, catalytic activities. We provide an overview of the current state of the art, and discuss the potential benefits of developing and using enzymes with genetically encoded non-canonical amino acids compared with enzymes containing only canonical amino acids.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalNature Catalysis
Volume3
Issue number3
DOIs
Publication statusPublished - 6-Jan-2020

Keywords

  • TRANSFER-RNA SYNTHETASE
  • RIBONUCLEOTIDE REDUCTASE
  • ESCHERICHIA-COLI
  • ARTIFICIAL METALLOENZYMES
  • COMPUTATIONAL DESIGN
  • GLOBAL INCORPORATION
  • OXIDASE ACTIVITY
  • CROSS-LINK
  • TYROSINE
  • SITE
  • unnatural amino acid
  • expanded genetic code
  • enzyme

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