Explantation of mesangial cell 'hillocks': a method for obtaining human mesangial cells in culture

E.W. Muller*, Y. Kim, A.F. Michael, R.L. Vernier, G.K. van der Hem, F.J. van der Woude

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    8 Citations (Scopus)

    Abstract

    A simple method is presented for selective cell culture of human mesangial cells using explanatation of mesangial cell hillocks. Glomeruli which had been incubated with collagenase were explanted on plastic tissue culture flasks. Three to 6 weeks after explantation, a rapidly growing multilayer of elongated mesangial cells was observed to grow over the previously established monolayer of glomerular epithelial cells, ultimately forming multiple nodular foci of mesangial cells or 'mesangial cell hillocks'. By explanting mesangial cell hillocks selectively, pure mesangial cell cultures were easily obtained. When compared with mesangial cells grown in mixed cultures from glomerular explants, the hillock-derived cells were identical in morphology, growth characteristics, cell markers and synthesis of extracellular matrix. This system provides a simple method for the isolation of human mesangial cells in culture.

    Original languageEnglish
    Pages (from-to)9-20
    Number of pages12
    JournalInternational Journal of Experimental Pathology
    Volume73
    Issue number1
    Publication statusPublished - Feb-1992

    Keywords

    • GLOMERULAR CULTURE
    • MESANGIAL CELLS
    • EXTRACELLULAR MATRIX
    • HUMAN GLOMERULAR CELLS
    • MONOCLONAL-ANTIBODIES
    • EPITHELIAL-CELLS
    • SMOOTH
    • PROTEINS
    • ANTIGENS
    • MATRIX
    • GROWTH

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