Exploring and validating innovative methods for detection and localization of head and neck squamous cell carcinoma primary tumors and lymph node metastases

    Research output: ThesisThesis fully internal (DIV)

    137 Downloads (Pure)

    Abstract

    Head and neck squamous cell carcinoma (HNSCC) is associated with a poor 5-year survival of less than 50%. New techniques could lead to improved detection of HNSCC and its metastases, resulting in a faster diagnosis. HNSCC can also be visualizated during an operation, which makes it easier to completely remove the tumor. Several such techniques are described in this thesis. Fluorescence imaging can be applied to lighten up a tumor intraoperatively, using antibodies bound to a fluorescent agent, which specifically target the tumor. Another technique is Narrow Band Imaging. This makes use of green and blue light, which better visualizes blood vessels and leads to improved detection of the aberrant blood vessel patterns seen in tumors. In oral cavity carcinoma, we have compared these two techniques to determine which may be best applied for tumor border assessment intraoperatively. Other studies described in this thesis focus on the search for more accurate antibodies for fluorescence imaging, in order to solely target the tumor and reduce background signal of healthy surrounding tissue, which are sometimes targeted as well. Moreover, studies for the detection of lymph node metastases were performed. According to standard of care, in case of suspicion of regional lymph node metastases, a fine-needle aspiration cytology is performed for diagnosis. Rather than microscopic examination, protein concentrations were measured in the aspiration samples and the feasibility for the detection of HNSCC was determined.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • University of Groningen
    Supervisors/Advisors
    • van der Laan, Bernard, Supervisor
    • Plaat, Boudewijn, Co-supervisor
    • van der Vegt, Bert, Co-supervisor
    Award date24-Oct-2022
    Place of Publication[Groningen]
    Publisher
    DOIs
    Publication statusPublished - 2022

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