Exploring the biocatalytic potential of a DyP-type peroxidase by profiling the substrate acceptance of Thermobifida fusca DyP peroxidase

Nikola Loncar, Dana I. Colpa, Marco W. Fraaije*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Dye-decolorizing peroxidases (DyPs) represent a new class of oxidative enzymes for which the natural substrates are largely unknown. To explore the biocatalytic potential of a DyP, we have studied the substrate acceptance profile of a robust DyP peroxidase, a DyP from Thermobifida fusca (TfuDyP). While previous work established that this bacterial peroxidase is able to act on a few reactive dyes and aromatic sulfides, this work significantly expands its substrate scope towards lignin related compounds, flavors, and various dyes. (C) 2016 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)7276-7281
Number of pages6
JournalTetrahedron
Volume72
Issue number46
DOIs
Publication statusPublished - 17-Nov-2016
Event12th Conference on Modern Developments in Biotransformations (Biotrans) - Vienna, Austria
Duration: 1-Jan-2015 → …

Keywords

  • Thermobifida fusca
  • Biocatalysis
  • Lignin
  • Peroxidase
  • Dye
  • Vanillin
  • RHODOCOCCUS-JOSTII RHA1
  • DECOLORIZING PEROXIDASE
  • HORSERADISH-PEROXIDASE
  • LIGNIN
  • DYES
  • IDENTIFICATION
  • DIVANILLIN
  • OXIDATION
  • ALCOHOL
  • FAMILY

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