Enzymology is a very large and interdisciplinary research field and enzymes have been the subject of intense research in order to unveil their functions in metabolism and their shortcomings leading to different diseases. Therefore, enzymes are a very common target in medicine and pharmaceutical industry research. What is interesting to note, they have been used since ancient times for the production of wine, cheese and bread through the use of yeast. At the end of the 20th century enzymology entered a new era of protein engineering. This was due to a spectacular development in determining protein structures, gene manipulation and sequencing, as well as in computing power and software that can predict the impact of mutations introduced to an enzyme. This also opened the road to enzyme design for industrial purposes and nowadays many enzymes have been successfully applied and still a lot of research effort is spent on further development in this field. This thesis was intended to study the structure, activity and mechanism of several oxidative enzymes. They all depend on a flavin cofactor which plays a pivotal role in the reactions they catalyse.
|Translated title of the contribution||Cofactor binding en biokatalytische eigenschappen van flavine bevattende enzymen|
|Qualification||Doctor of Philosophy|
|Publication status||Published - 2014|