Expression and inheritance of inserted markers in binary vector carrying Agrobacterium rhizogenes-transformed potato (Solanum tuberosum L.)

R.G.F. Visser*, A. Hesseling-Meinders, E. Jacobsen, H Nijdam, B. Witholt, W.J. Feenstra

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    Transgenic shoots were regenerated from eight
    diploid potato hairy root clones obtained by transformation with Agrobacterium rhizogenes harboring next to its
    wild-type Ri-plasmid a binary vector containing the
    neomycin phosphotransferase and the fl-glucuronidase
    genes. The plants exhibited the typical hairy root phenotype. Of the plants isolated, 58% were tetraploid and
    38 % were diploid. Flowering and tuberization was much
    better in the diploid than in the tetraploid plants. Transgenic plants formed a significantly larger root system
    when grown on kanamycin-containing medium as compared to growth on kanamycin-free medium. Direct evidence for genetic transformation was obtained by opine,
    neomycin phosphotransferase and fl-glucuronidase assays, and by molecular hybridization. Fourteen flowering diploid plants were reciprocally crossed with untransformed S. tuberosum plants, but only six were successful.
    Seedlings obtained from four crosses showed that all
    traits were transmitted to the offspring. Molecular analysis confirmed the presence of multiple integrations
    (copies) of both vector T-DNA and Ri-T-DNA. The
    genetic data, furthermore, suggest that the traits derived
    from Ri-T-DNA and binary vector T-DNA are linked,
    as no recombination between the different traits was observed.
    Original languageEnglish
    Pages (from-to)705-714
    Number of pages10
    JournalTheoretical and Applied Genetics
    Volume78
    Issue number5
    DOIs
    Publication statusPublished - 1989

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