Expression of interleukin-1 beta in rat dorsal root ganglia

JCVM Copray*, [No Value] Mantingh, N Brouwer, K Biber, BM Kust, RSB Liem, [No Value] Huitinga, FJH Tilders, AM Van Dam, HWGM Boddeke

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

110 Citations (Scopus)

Abstract

The expression of interleukin-lp was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in Situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1 beta mRNA: about 80% of these DRG neurons actually appeared to produce the IL-lp protein at markedly varying levels. The expression of IL-1 beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1 beta immunopositive sensory neurons included most of the large calretinin-positive la afferents. but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 beta receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1 beta in the DRG neurons needs to be elucidated, but we speculate that IL-1 beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission. (C) 2001 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalJournal of Neuroimmunology
Volume118
Issue number2
Publication statusPublished - 30-Aug-2001

Keywords

  • interleukin-1 beta
  • sensory neurons
  • type 1 interleukin-1 receptor
  • dorsal root ganglion
  • IMMUNODEFICIENCY-VIRUS TYPE-1
  • PRIMARY AFFERENT NEURONS
  • NERVOUS-SYSTEM
  • MESSENGER-RNA
  • SPINAL-CORD
  • IMMUNOREACTIVE INTERLEUKIN-1-BETA
  • RECEPTOR ANTAGONIST
  • HIPPOCAMPAL-NEURONS
  • MULTIPLE-SCLEROSIS
  • RAMIFIED MICROGLIA

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