Expression of the transporter encoded by the cefT gene of Acremonium chrysogenum increases cephalosporin production in Penicillium chrysogenum

Jeroen G. Nijland, Andriy Kovalchuk, Marco A. van den Berg, Roel A. L. Bovenberg, Arnold J. M. Driessen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)
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Abstract

By introduction of the cefEF genes of Acremonium chrysogenum and the cmcH gene of Streptomyces clavuligerus, Penicillium chrysogenum can be reprogrammed to form adipoyl-7-amino-3-carbamoyloxymethyl-3-cephem-4-carboxylic acid (ad7-ACCCA), a carbamoylated derivate of adipoyl-7-aminodeacetoxy-cephalosporanic acid. The cefEF gene of A. chrysogenum encodes a cephalosporin C transporter that belongs to the Major Facilitator Superfamily. Introduction of cefT into an ad7-ACCCA-producing P. chrysogenum strain results in an almost 2-fold increase in cephalosporin production with a concomitant decrease in penicillin by-product formation. These data suggest that cephalosporin production by recombinant P. chrysogenum strains is limited by the ability of the fungus to secrete these compounds. (c) 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1415-1421
Number of pages7
JournalFungal Genetics and Biology
Volume45
Issue number10
DOIs
Publication statusPublished - Oct-2008

Keywords

  • Cephalosporin biosynthesis
  • Transport
  • Penicillium
  • Acremonium
  • RING EXPANSION
  • C PRODUCTION
  • PROTEIN
  • ACID

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