Extracellular Adenosine Triphosphate Affects Systemic and Kidney Immune Cell Populations in Pregnant Rats

Floor Spaans*, Barbro N. Melgert, Theo Borghuis, Pieter A. Klok, Paul de Vos, Winston W. Bakker, Harry van Goor, Marijke Faas

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

PROBLEM: Changes in the systemic immune response are found in preeclampsia. This may be related to high extracellular adenosine triphosphate (ATP) levels. The question arose whether ATP could affect immune responses in pregnancy. Previously, we investigated whether ATP affected monocyte activation and subpopulations. Here, we investigated ATP-induced changes in other immune cell populations in pregnant rats, systemically and in the kidney, an affected organ in preeclampsia.

METHOD OF STUDY: Using flow cytometry or immunohistochemistry, blood and kidney leukocytes were studied in pregnant and non-pregnant rats at different intervals after ATP or saline infusion.

RESULTS: Adenosine triphosphate (ATP) infusion induced increased peripheral blood non-classical monocytes and decreased T lymphocyte subsets in pregnant rats only, higher glomerular macrophage and T lymphocyte numbers in non-pregnant animals 1 day after infusion, and higher glomerular macrophage numbers in pregnant rats 6 days after infusion.

CONCLUSION: Adenosine triphosphate (ATP) infusion in pregnant rats induced a pregnancy-specific inflammatory response. Increased ATP levels could potentially contribute to development of the inflammatory response of preeclampsia.

Original languageEnglish
Pages (from-to)305-316
Number of pages12
JournalAmerican Journal of Reproductive Immunology
Volume72
Issue number3
Early online date8-May-2014
DOIs
Publication statusPublished - Sep-2014

Keywords

  • Extracellular ATP
  • kidney
  • leukocytes
  • preeclampsia
  • rat model
  • REGULATORY T-CELLS
  • LOW-DOSE ENDOTOXIN
  • EXPERIMENTAL PREECLAMPSIA
  • PERIPHERAL-BLOOD
  • PORE FORMATION
  • HUMAN PLACENTA
  • ATP
  • RECEPTOR
  • MONOCYTE
  • ACTIVATION

Cite this