Fabrication and Mechanical properties of Supercharged polypeptides based Biomaterials: from Adhesives to Fibers

Jing Sun

Research output: ThesisThesis fully internal (DIV)

372 Downloads (Pure)

Abstract

High-tech and environmentally friendly biomaterials are emerging and rapidly expanding class of materials and have been widely applied in the biomedical field. Among the promising body of biopolymers, proteins with their unique structural and biological properties are being exploited extensively for the creation of innovative biomaterials, such as adhesives and fibers. The overall goal of the project was to produce protein-based mechanical biomaterials using bioengineered proteins as a platform. In this thesis, various biomaterials have been produced by relying on electrostatic interactions between supercharged polypeptides (SUPs) and surfactants, including SUP glues and SUP fibers. SUPs are inspired by natural elastin and were expressed recombinantly in E. Coli. SUPs mainly consist of repetitive pentapeptide sequences (VPGXG)n, in which the fourth position X represents either lysine (K) or glutamic acid (E). By the choice of these two amino acids positive and negative charges are introduced into the polypeptide backbone, respectively. Chapter 1 presents a brief introduction to protein-based biomaterials with extraordinary mechanical properties, ranging from protein-based glues to protein-based fibers. Chapters 2, 3, and 4 focus on the fabrication and application of SUP glues. Chapter 5 aims to develop SUP fibers with mechanical behaviors that can be modulated reversibly by light. Finally, some conclusive remarks for SUP-based biomaterials are provided in Chapter 6.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
Supervisors/Advisors
  • Herrmann, Andreas, Supervisor
  • Portale, Giuseppe, Co-supervisor
  • Kamperman, M.M.G., Assessment committee, External person
  • Hest, Jan C.M. van, Assessment committee, External person
  • Peneva, Kalina, Assessment committee, External person
Award date10-Feb-2020
Place of Publication[Groningen]
Publisher
Print ISBNs978-94-034-2435-4
Electronic ISBNs978-94-034-2435-7
DOIs
Publication statusPublished - 2020

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