FGF-2-loaded collagen scaffolds attract cells and blood vessels in rat oral mucosa

Richard G. Jansen, Toin H. van Kuppevelt, Willeke F. Daamen, Anne M. Kuijpers-Jagtman, Johannes W. Von den Hoff*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Background:

Wound contraction and scar formation after cleft palate repair impair the growth of the maxilla. The implantation of a growth factor-loaded scaffold might solve these problems.

Methods:

The tissue response to fibroblast growth factor (FGF)-2 loaded collagen scaffolds was evaluated after implantation in the palate of rats. Scaffolds, with and without FGF-2, were implanted submucoperiosteally in the palate of 25 rats and evaluated after up to 16 weeks. On hematoxylin and eosin (H&E)-stained sections, the cell density and the number of giant cells within the scaffolds were quantified. Infiltration of inflammatory cells, myofibroblasts, and the number of blood vessels were quantified after immunohistochemistry.

Results:

The cell density was significantly higher in the FGF-2 group up to 4 weeks after implantation (102% at 2 weeks, P < 0.001). The number of blood vessels was also significantly higher in the FGF-2 group at 1 and 2 weeks (316% at 1 week, P = 0.003), but the myofibroblast score was lower (100% at 2 weeks, P = 0.008). A comparable mild and rapidly subsiding inflammatory response and foreign body reaction were found in both groups.

Conclusion:

FGF-2-loaded scaffolds displayed a faster influx of host cells, an increased rate of vascularization, and a reduced differentiation of myofibroblasts. These scaffolds might therefore be highly suitable for intra-oral reconstructions, such as cleft palate repair.

Original languageEnglish
Pages (from-to)630-638
Number of pages9
JournalJournal of oral pathology & medicine
Volume38
Issue number8
DOIs
Publication statusPublished - Sept-2009
Externally publishedYes

Keywords

  • FGF-2
  • in vivo test
  • oral
  • scaffold
  • wound healing
  • FIBROBLAST-GROWTH-FACTOR
  • SMOOTH MUSCLE ACTIN
  • WOUND CONTRACTION
  • TISSUE REGENERATION
  • INCISIONAL WOUNDS
  • PALATAL MUCOSA
  • FACTOR BFGF
  • SKIN
  • ANGIOGENESIS
  • EXPRESSION

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