Fiber-type-specific sensitivities and phenotypic adaptations to dietary fat overload differentially impact fast- versus slow-twitch muscle contractile function in C57BL/6J mice

Jolita Ciapaite*, Sjoerd A. van den Berg, Sander M. Houten, Klaas Nicolay, Ko Willems van Dijk, Jeroen Jeneson

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    46 Citations (Scopus)

    Abstract

    High-fat diets (HFDs) have been shown to interfere with skeletal muscle energy metabolism and cause peripheral insulin resistance. However, understanding of HFD impact on skeletal muscle primary function, i.e., contractile performance, is limited. Male C57BL/6J mice were fed HFD containing lard (HFL) or palm oil (HFP), or low-fat diet (LED) for 5 weeks. Fast-twitch (FT) extensor digitorum Iongus (EDL) and slow-twitch (ST) soleus muscles were characterized with respect to contractile function and selected biochemical features. In EDL muscle, a 30%-50% increase in fatty acid (FA) content and doubling of long-chain acylcarnitine (C14-C18) content in response to HFL and HFP feeding were accompanied by increase in protein levels of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha, mitochondrial oxidative phosphorylation complexes and acyl-CoA dehydrogenases involved in mitochondrial FA beta-oxidation. Peak force of FT EDL twitch and tetanic contractions was unaltered, but the relaxation time (RT) of twitch contractions was 30% slower compared to LFD controls. The latter was caused by accumulation of lipid intermediates rather than changes in the expression levels of proteins involved in calcium handling. In ST soleus muscle, no evidence for lipid overload was found in any HFD group. However, particularly in HFP group, the peak force of twitch and tetanic contractions was reduced, but RT was faster than LFD controls. The latter was associated with a fast-to-slow shift in troponin T isoform expression. Taken together, these data highlight fiber-type-specific sensitivities and phenotypic adaptations to dietary lipid overload that differentially impact fast- versus slow-twitch skeletal muscle contractile function. (C) 2015 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)155-164
    Number of pages10
    JournalJournal of Nutritional Biochemistry
    Volume26
    Issue number2
    DOIs
    Publication statusPublished - Feb-2015

    Keywords

    • High-fat-diet-induced obesity
    • Skeletal muscle function
    • Fiber type
    • Fatty acid metabolism
    • Oxidative phosphorylation
    • Troponin T
    • CHAIN ACYL-COA
    • SKELETAL-MUSCLE
    • INSULIN-RESISTANCE
    • OXIDATIVE CAPACITY
    • TROPONIN-T
    • SARCOPLASMIC-RETICULUM
    • FED RATS
    • METABOLISM
    • MITOCHONDRIA
    • ISOFORMS

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