Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment

Meng Zhang; Fenghua Zhao; Xue Zhang; Linda A Brouwer; Janette K Burgess; Martin C Harmsen

doi:10.1016/j.mtbio.2023.100842

Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment

Meng Zhang, Fenghua Zhao, Xue Zhang, Linda A Brouwer, Janette K Burgess, Martin C Harmsen

Groningen Research Institute for Asthma and COPD (GRIAC)

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Abstract

This study aimed to investigate the impact of fibroblasts (MRC-5) on the extracellular matrix (ECM) microenvironment of endothelial cells (ECs) during the vascularization of skin-derived ECM hydrogel in vitro. Two types of ECs were studied: human dermal microvascular endothelial cells (HMEC) and human pulmonary microvascular endothelial cells (HPMEC). Results showed that the presence of MRC-5 fibroblasts increased the stiffness of the hydrogel and led to larger fiber diameters and increased porosity. Extensive collagen fiber remodeling occurred in the ECM hydrogel with MRC-5 fibroblasts. Additionally, higher levels of fibulin-1 and fibronectin were deposited in the hydrogel when co-cultured with MRC-5 fibroblasts. These findings suggest that MRC-5 fibroblasts play a role in modifying the ECM microenvironment, promoting vascularization through dynamic ECM remodeling.

Original language	English
Article number	100842
Number of pages	13
Journal	Materials Today Bio
Volume	23
DOIs	https://doi.org/10.1016/j.mtbio.2023.100842
Publication status	Published - Dec-2023

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Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironmentFinal publisher's version, 17.3 MBLicence: CC BY

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@article{887eb705130a4bbfb61f6b980fcb2ffa,

title = "Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment",

abstract = "This study aimed to investigate the impact of fibroblasts (MRC-5) on the extracellular matrix (ECM) microenvironment of endothelial cells (ECs) during the vascularization of skin-derived ECM hydrogel in vitro. Two types of ECs were studied: human dermal microvascular endothelial cells (HMEC) and human pulmonary microvascular endothelial cells (HPMEC). Results showed that the presence of MRC-5 fibroblasts increased the stiffness of the hydrogel and led to larger fiber diameters and increased porosity. Extensive collagen fiber remodeling occurred in the ECM hydrogel with MRC-5 fibroblasts. Additionally, higher levels of fibulin-1 and fibronectin were deposited in the hydrogel when co-cultured with MRC-5 fibroblasts. These findings suggest that MRC-5 fibroblasts play a role in modifying the ECM microenvironment, promoting vascularization through dynamic ECM remodeling.",

author = "Meng Zhang and Fenghua Zhao and Xue Zhang and Brouwer, {Linda A} and Burgess, {Janette K} and Harmsen, {Martin C}",

note = "{\textcopyright} 2023 The Authors.",

year = "2023",

month = dec,

doi = "10.1016/j.mtbio.2023.100842",

language = "English",

volume = "23",

journal = "Materials Today Bio",

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publisher = "Elsevier",

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TY - JOUR

T1 - Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment

AU - Zhang, Meng

AU - Zhao, Fenghua

AU - Zhang, Xue

AU - Brouwer, Linda A

AU - Burgess, Janette K

AU - Harmsen, Martin C

PY - 2023/12

Y1 - 2023/12

N2 - This study aimed to investigate the impact of fibroblasts (MRC-5) on the extracellular matrix (ECM) microenvironment of endothelial cells (ECs) during the vascularization of skin-derived ECM hydrogel in vitro. Two types of ECs were studied: human dermal microvascular endothelial cells (HMEC) and human pulmonary microvascular endothelial cells (HPMEC). Results showed that the presence of MRC-5 fibroblasts increased the stiffness of the hydrogel and led to larger fiber diameters and increased porosity. Extensive collagen fiber remodeling occurred in the ECM hydrogel with MRC-5 fibroblasts. Additionally, higher levels of fibulin-1 and fibronectin were deposited in the hydrogel when co-cultured with MRC-5 fibroblasts. These findings suggest that MRC-5 fibroblasts play a role in modifying the ECM microenvironment, promoting vascularization through dynamic ECM remodeling.

AB - This study aimed to investigate the impact of fibroblasts (MRC-5) on the extracellular matrix (ECM) microenvironment of endothelial cells (ECs) during the vascularization of skin-derived ECM hydrogel in vitro. Two types of ECs were studied: human dermal microvascular endothelial cells (HMEC) and human pulmonary microvascular endothelial cells (HPMEC). Results showed that the presence of MRC-5 fibroblasts increased the stiffness of the hydrogel and led to larger fiber diameters and increased porosity. Extensive collagen fiber remodeling occurred in the ECM hydrogel with MRC-5 fibroblasts. Additionally, higher levels of fibulin-1 and fibronectin were deposited in the hydrogel when co-cultured with MRC-5 fibroblasts. These findings suggest that MRC-5 fibroblasts play a role in modifying the ECM microenvironment, promoting vascularization through dynamic ECM remodeling.

U2 - 10.1016/j.mtbio.2023.100842

DO - 10.1016/j.mtbio.2023.100842

M3 - Article

C2 - 37942422

SN - 2590-0064

VL - 23

JO - Materials Today Bio

JF - Materials Today Bio

M1 - 100842

ER -

Fibroblasts alter the physical properties of dermal ECM-derived hydrogels to create a pro-angiogenic microenvironment

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