Fibrosis impairs the formation of new myofibers in the soft palate after injury

Paola L. Carvajal Monroy, Sander Grefte, Anne Marie Kuijpers-Jagtman, Maria P. A. C. Helmich, Frank A. D. T. G. Wagener, Johannes W. von den Hoff*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Muscle repair is a crucial component of palatoplasty but little is known about muscle regeneration after cleft palate repair. We hypothesized that the formation of new myofibers is hampered by collagen accumulation after experimental injury of the soft palate of rats. One-millimeter excisional defects were made in the soft palates of 32 rats. The wound area was evaluated after 3, 7, 28, and 56 days using azocarmine G and aniline blue to stain for collagen and immunohistochemistry to identify myofibroblasts and to monitor skeletal muscle differentiation. To evaluate age effects, 16 unwounded animals were evaluated at 3 and 56 days. Staining was quantified by image analysis, and one-way ANOVA was used for the statistical analysis. At day 56, the area percentage of collagen-rich tissue was higher in the injured palatal muscles (46.7 +/- 6.9%) than in nonwounded controls (15.9 +/- 1.0%, p

Original languageEnglish
Pages (from-to)866-873
Number of pages8
JournalWound repair and regeneration
Volume23
Issue number6
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • MUSCLE SATELLITE CELLS
  • SKELETAL-MUSCLE
  • CLEFT-PALATE
  • VELOPHARYNGEAL INSUFFICIENCY
  • CARDIOTOXIN INJURY
  • MYOBLAST FUSION
  • BASAL LAMINA
  • REGENERATION
  • REPAIR
  • MICE

Cite this