Fibrosis impairs the formation of new myofibers in the soft palate after injury

Paola L.  Carvajal Monroy; Sander Grefte; Anne Marie Kuijpers-Jagtman; Maria P. A. C. Helmich; Frank A. D. T. G. Wagener; Johannes W. von den Hoff

doi:10.1111/wrr.12345

Fibrosis impairs the formation of new myofibers in the soft palate after injury

Paola L. Carvajal Monroy, Sander Grefte, Anne Marie Kuijpers-Jagtman, Maria P. A. C. Helmich, Frank A. D. T. G. Wagener, Johannes W. von den Hoff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Muscle repair is a crucial component of palatoplasty but little is known about muscle regeneration after cleft palate repair. We hypothesized that the formation of new myofibers is hampered by collagen accumulation after experimental injury of the soft palate of rats. One-millimeter excisional defects were made in the soft palates of 32 rats. The wound area was evaluated after 3, 7, 28, and 56 days using azocarmine G and aniline blue to stain for collagen and immunohistochemistry to identify myofibroblasts and to monitor skeletal muscle differentiation. To evaluate age effects, 16 unwounded animals were evaluated at 3 and 56 days. Staining was quantified by image analysis, and one-way ANOVA was used for the statistical analysis. At day 56, the area percentage of collagen-rich tissue was higher in the injured palatal muscles (46.7 +/- 6.9%) than in nonwounded controls (15.9 +/- 1.0%, p

Original language	English
Pages (from-to)	866-873
Number of pages	8
Journal	Wound repair and regeneration
Volume	23
Issue number	6
DOIs	https://doi.org/10.1111/wrr.12345
Publication status	Published - 2015
Externally published	Yes

Keywords

MUSCLE SATELLITE CELLS
SKELETAL-MUSCLE
CLEFT-PALATE
VELOPHARYNGEAL INSUFFICIENCY
CARDIOTOXIN INJURY
MYOBLAST FUSION
BASAL LAMINA
REGENERATION
REPAIR
MICE

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@article{7fb1db545afb4502a51bdd8a35abf7e8,

title = "Fibrosis impairs the formation of new myofibers in the soft palate after injury",

abstract = "Muscle repair is a crucial component of palatoplasty but little is known about muscle regeneration after cleft palate repair. We hypothesized that the formation of new myofibers is hampered by collagen accumulation after experimental injury of the soft palate of rats. One-millimeter excisional defects were made in the soft palates of 32 rats. The wound area was evaluated after 3, 7, 28, and 56 days using azocarmine G and aniline blue to stain for collagen and immunohistochemistry to identify myofibroblasts and to monitor skeletal muscle differentiation. To evaluate age effects, 16 unwounded animals were evaluated at 3 and 56 days. Staining was quantified by image analysis, and one-way ANOVA was used for the statistical analysis. At day 56, the area percentage of collagen-rich tissue was higher in the injured palatal muscles (46.7 +/- 6.9%) than in nonwounded controls (15.9 +/- 1.0%, p",

keywords = "MUSCLE SATELLITE CELLS, SKELETAL-MUSCLE, CLEFT-PALATE, VELOPHARYNGEAL INSUFFICIENCY, CARDIOTOXIN INJURY, MYOBLAST FUSION, BASAL LAMINA, REGENERATION, REPAIR, MICE",

author = "{Carvajal Monroy}, {Paola L.} and Sander Grefte and Kuijpers-Jagtman, {Anne Marie} and Helmich, {Maria P. A. C.} and Wagener, {Frank A. D. T. G.} and {von den Hoff}, {Johannes W.}",

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language = "English",

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T1 - Fibrosis impairs the formation of new myofibers in the soft palate after injury

AU - Carvajal Monroy, Paola L.

AU - Grefte, Sander

AU - Kuijpers-Jagtman, Anne Marie

AU - Helmich, Maria P. A. C.

AU - Wagener, Frank A. D. T. G.

AU - von den Hoff, Johannes W.

PY - 2015

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N2 - Muscle repair is a crucial component of palatoplasty but little is known about muscle regeneration after cleft palate repair. We hypothesized that the formation of new myofibers is hampered by collagen accumulation after experimental injury of the soft palate of rats. One-millimeter excisional defects were made in the soft palates of 32 rats. The wound area was evaluated after 3, 7, 28, and 56 days using azocarmine G and aniline blue to stain for collagen and immunohistochemistry to identify myofibroblasts and to monitor skeletal muscle differentiation. To evaluate age effects, 16 unwounded animals were evaluated at 3 and 56 days. Staining was quantified by image analysis, and one-way ANOVA was used for the statistical analysis. At day 56, the area percentage of collagen-rich tissue was higher in the injured palatal muscles (46.7 +/- 6.9%) than in nonwounded controls (15.9 +/- 1.0%, p

AB - Muscle repair is a crucial component of palatoplasty but little is known about muscle regeneration after cleft palate repair. We hypothesized that the formation of new myofibers is hampered by collagen accumulation after experimental injury of the soft palate of rats. One-millimeter excisional defects were made in the soft palates of 32 rats. The wound area was evaluated after 3, 7, 28, and 56 days using azocarmine G and aniline blue to stain for collagen and immunohistochemistry to identify myofibroblasts and to monitor skeletal muscle differentiation. To evaluate age effects, 16 unwounded animals were evaluated at 3 and 56 days. Staining was quantified by image analysis, and one-way ANOVA was used for the statistical analysis. At day 56, the area percentage of collagen-rich tissue was higher in the injured palatal muscles (46.7 +/- 6.9%) than in nonwounded controls (15.9 +/- 1.0%, p

KW - MUSCLE SATELLITE CELLS

KW - SKELETAL-MUSCLE

KW - CLEFT-PALATE

KW - VELOPHARYNGEAL INSUFFICIENCY

KW - CARDIOTOXIN INJURY

KW - MYOBLAST FUSION

KW - BASAL LAMINA

KW - REGENERATION

KW - REPAIR

KW - MICE

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JO - Wound repair and regeneration

JF - Wound repair and regeneration

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ER -