Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell

Luca Mantovanelli, Bauke F. Gaastra, Bert Poolman*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Abstract

Fluorescence-based sensors play a fundamental role in biological research. These sensors can be based on fluorescent proteins, fluorescent probes or they can be hybrid systems. The availability of a very large dataset of fluorescent molecules, both genetically encoded and synthetically produced, together with the structural insights on many sensing domains, allowed to rationally design a high variety of sensors, capable of monitoring both molecular and global changes in living cells or in in vitro systems. The advancements in the fluorescence-imaging field helped researchers to obtain a deeper understanding of how and where specific changes occur in a cell or in vitro by combining the readout of the fluorescent sensors with the spatial information provided by fluorescent microscopy techniques. In this review we give an overview of the state of the art in the field of fluorescent biosensors and fluorescence imaging techniques, and eventually guide the reader through the choice of the best combination of fluorescent tools and techniques to answer specific biological questions. We particularly focus on sensors for probing the bioenergetics and physicochemical status of the cell.

Original languageEnglish
Title of host publicationCurrent Topics in Membranes
Subtitle of host publicationNew Methods and Sensors for Membrane and Cell Volume Research
EditorsMichael A. Model, Irena Levitan
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Chapter1
Pages1-54
Number of pages54
Volume88
ISBN (Print)978-0-323-91114-6
DOIs
Publication statusPublished - 2021

Publication series

NameCurrent Topics in Membranes
ISSN (Print)1063-5823

Keywords

  • Bioenergetics
  • Fluorescence
  • Fluorescent biosensensors
  • FRET
  • Microscopic imaging
  • Physicochemistry of cell
  • Ratiometric and intensiometric fluorophores

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