Fluticasone uptake across Calu-3 cells is mediated by salmeterol when deposited as a combination powder inhaler

Mehra Haghi, Daniela Traini, Dirkje S. Postma, Mary Bebawy, Paul M. Young*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Background and objectiveWe assessed whether co-deposition of a long-acting (2)-agonist and a corticosteroid affects their respective transport rates across epithelial cells.

MethodsDrug particles were deposited on the air-interface culture of Calu-3 cells using a twin-stage impinger. We compared the transport rate of salmeterol and fluticasone across the epithelial cells using commercially available formulations (Serevent, Flixotide and Seretide). The transepithelial resistance of Calu-3 cells was measured before and after each deposition to monitor epithelial resistance.

ResultsThe codeposition of salmeterol and fluticasone had no significant effect on transport of salmeterol through the cell layer. In contrast, the rate of fluticasone propionate transport in presence of salmeterol xinofoate was significantly lower (0.530.20%) compared with the single fluticasone formulation (2.360.97%). Furthermore, the resistance of the epithelial cells was significantly increased after salmeterol deposition from both single and combination products.

ConclusionsOur data demonstrate that salmeterol may decrease the permeability of epithelial cells, resulting in slower fluticasone transport across Calu-3 epithelial monolayers. The subsequent increased residence time of fluticasone in the airways could prolong its anti-inflammatory effects.

Original languageEnglish
Pages (from-to)1197-1201
Number of pages5
JournalRespirology
Volume18
Issue number8
DOIs
Publication statusPublished - Nov-2013

Keywords

  • Calu-3
  • epithelial transport
  • fluticasone propionate
  • salmeterol xinofoate
  • PROPIONATE
  • ASTHMA
  • CORTICOSTEROIDS
  • PERMEABILITY
  • DELIVERY
  • MODEL
  • LUNG

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