Foreign-body reaction to dermal sheep collagen in interferon-gamma-receptor knock-out mice

IMSL Khouw*, PB van Wachem, JA Plantinga, BL Haagmans, LFMH de Leij, MJA van Luyn

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

This study was performed to gain more insight into the role of interferon-gamma (IFN-gamma), a potent macrophage activator, in the foreign-body reaction to hexamethylenediisocyanate-crosslinked dermal sheep collagen (HDSC). Because the results of earlier studies aimed at modulating the foreign-body reaction in AO rats by local or systemic treatment with anti-IFN-gamma were not completely unambiguous, we extended our in investigations to IFN-gamma-receptor knock-out (KO) mice. Several parameters (i.e., macrophages, giant cells, T-cells, B-cells, granulocytes, expression of MHC class II, stroma formation, and degradation and calcification of the biomaterial) were compared between wild-type (WT) and KO mice. Remarkably, the foreign-body reaction was very similar in WT and KO mice. in both, giant cells were formed, but in contrast to previous results in AO rats, phagocytosis of HDSC bundles occurred hardly at all up to 9 weeks, and MHC class II expression was minimal. Stroma formation and vascularization were high and calcification occurred. T-cells comprised less than 1%; a few plasma cells were present in the KO mice at later time points. Granulocytes, mainly eosinophils, were present at all explantation time points. Because of the similar results in WT and KO mice, we question whether IFN-gamma plays a role at all in the foreign-body reaction in mice. (C) 2000 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalJournal of Biomedical Materials Research
Volume50
Issue number2
Publication statusPublished - May-2000

Keywords

  • foreign-body reaction
  • IFN-gamma-receptor knockout mice
  • macrophages and giant cells
  • MHC class II
  • MONOCLONAL-ANTIBODY GK1.5
  • T-CELL
  • TISSUE REACTION
  • IFN-GAMMA
  • IN-VIVO
  • MONOCYTES MACROPHAGES
  • LEISHMANIA-MAJOR
  • IMMUNE-RESPONSE
  • DEFICIENT MICE
  • CROSS-LINKING

Cite this