Frat is dispensable for canonical Wnt signaling in mammals

Renée van Amerongen, Martijn Nawijn, Jonathan Franca-Koh, John Zevenhoven, Hanneke van der Gulden, Jos Jonkers, Anton Berns

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Abstract

Wnt-signal transduction through beta-catenin is thought to require the inhibition of GSK3 by Frat/GBP. To investigate the role of Frat in mammalian development, we have generated mice with targeted mutations in all three murine Frat homologs. We show that Frat is normally expressed at sites of active Wnt signaling. Surprisingly' Frat-deficient mice do not display gross abnormalities' Moreover, canonical Wnt signaling in primary cells is unaffected by the loss of Frat. These studies show that Frat is not an essential component of the canonical Wnt pathway in higher organisms, despite the strict requirement of Frat/GBP for maternal Wnt signaling in Xenopus.

Original languageEnglish
Pages (from-to)425-430
Number of pages6
JournalGenes & Development
Volume19
Issue number4
DOIs
Publication statusPublished - 15-Feb-2005

Keywords

  • Frat
  • GBP
  • Wnt
  • knockout
  • beta-catenin
  • glycogen synthase kinase 3
  • BETA-CATENIN
  • INTESTINAL EPITHELIUM
  • SOCIAL-INTERACTION
  • MOLECULAR-CLONING
  • XENOPUS EMBRYOS
  • AXIS FORMATION
  • EXPRESSION
  • TRANSCRIPTION
  • GENE
  • PROLIFERATION

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