Functional expression of the fractalkine (CX3C) receptor and its regulation by lipopolysaccharide in rat microglia

EWGH Boddeke*, [No Value] Meigel, S Frentzel, K Biber, LQ Renn, P Gebicke-Harter

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

67 Citations (Scopus)

Abstract

Functional expression of CX,CRI, a recently discovered receptor for the chemokine fractalkine, was investigated in cultured rat microglia. Reverse transcriptase polymerase chain reaction (PCR) experiments show abundant expression of fractalkine receptor mRNA in microglia. mRNA expression of fractalkine was undetectable in astrocytes and microglia but was very strong in cortical neurons Incubation of microglia with lipopolysaccharide (100 ng/ml) transiently suppressed expression of fractalkine receptor mRNA. Fractalkine induced a concentration-dependent (10(-10)-10(-8) M) and, at high concentrations, oscillatory mobilization of intracellular Ca2+ in microglia The concentration-response curve of fractalkine was shifted to the right after 12 h incubation with lipopolysaccharide. It is concluded that treatment with endotoxin downregulates expression of fractalkine receptor mRNA in rat microglia and suppresses the functional response to fractalkine. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalEuropean Journal of Pharmacology
Volume374
Issue number2
Publication statusPublished - 18-Jun-1999

Keywords

  • fractalkine
  • CX3C1 receptor
  • lipopolysaccharide
  • MESSENGER-RNA
  • BRAIN
  • CHEMOKINE
  • STIMULATION
  • ENDOTOXIN
  • CELLS

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