Gas Chromatographic Determination of N-acetylisoputreanine-gamma-lactam, a Unique Catabolite of N1-acetylspermidine

J Hessels*, A.W Kingma, M.C.J.M Sturkenboom, H Elzinga, G.A van den Berg , F.A.J Muskiet

*Corresponding author for this work

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    Abstract

    A capillary gas chromatographic method with nitrogen-phosphorus detection for the determination of N-acetylisoputreanine-gamma-lactam (acisoga) in urine is described. The method was validated by comparing the results with those given by an isotope dilution mass fragmentographic method. Making use of specific inhibitors for copper-dependent amine oxidase and polyamine oxidase in rats, it was demonstrated that acisoga is formed by oxidative deamination of N1-acetylspermidine by the former enzyme. Moreover, acisoga is not a substrate for pig liver polyamine oxidase. Increased concentrations of acisoga, relative to N1-acetylspermidine, in urines of patients with non-Hodgkin's lymphoma indicated that this conversion diminishes the sensitivity of N1-acetylspermidine as a marker for (tumour) cell death.

    Original languageEnglish
    Pages (from-to)1-9
    Number of pages9
    JournalJournal of chromatography-Biomedical applications
    Volume563
    Issue number1
    DOIs
    Publication statusPublished - 18-Jan-1991

    Keywords

    • POLYAMINE OXIDASE
    • RAT-LIVER
    • SPERMIDINE
    • URINE
    • METABOLITES

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