Gene cluster for biosynthesis of thermophilin 1277-a lantibiotic produced by Streptococcus thermophilus SBT1277, and heterologous expression of TepI, a novel immunity peptide

T. Kabuki*, Y. Kawai, H. Uenishi, Y. Seto, J. Kok, H. Nakajima, T. Saito

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Aims:

To identify genes cluster for thermophilin 1277 produced by Streptococcus thermophilus SBT1277.

Methods and Results:

To identify genes for thermophilin 1277 production, the chromosomal DNA region surrounding the structural gene, tepA, was sequenced using a primer-walking method. The thermophilin 1277 biosynthesis gene locus (tep) is a 9 center dot 9-kb region, which consists of at least ten open reading frames (ORFs) in the following order: tepAMTFEGKRI and ORF4. Homology analysis showed high similarity to genes involved in bovicin HJ50 production by Streptococcus bovis HJ50. tepI encodes a novel, small, positively charged hydrophobic peptide of 52 amino acids, which contains a putative transmembrane segment. By heterologous expression in Lactococcus lactis ssp. cremoris MG1363, the TepI-expressing strain exhibited at least 1 center dot 3 times higher resistance to thermophilin 1277.

Conclusions:

Thermophilin 1277 biosynthesis genes were encoded by a 9 center dot 9-kbp region containing at least ten ORFs. TepI is a novel immunity peptide, which protected Strep. thermophilus SBT1277 against thermophilin 1277 in addition to TepFEG, a putative ABC transporter.

Significance and Impact of the Study:

This is the first report regarding a lantibiotic gene cluster produced by Strep. thermophilus strain.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalJournal of Applied Microbiology
Volume110
Issue number3
DOIs
Publication statusPublished - Mar-2011

Keywords

  • AII type
  • bacteriocin
  • immunity peptide
  • lantibiotic
  • operon
  • Streptococcus thermophilus
  • HOST BACILLUS-SUBTILIS
  • GRAM-POSITIVE BACTERIA
  • FUNCTIONAL-ANALYSIS
  • LACTOCOCCUS-LACTIS
  • LACTICIN-481 SYNTHETASE
  • ABC TRANSPORTERS
  • STRUCTURAL GENE
  • NISIN IMMUNITY
  • BOVICIN HJ50
  • MUTACIN II

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