Generation and Application of Inducible Chimeric RNA ASTN2-PAPPA(as) Knockin Mouse Model

Yichen Luo; Liang Du; Zhimeng Yao; Fan Liu; Kai Li; Feifei Li; Jianlin Zhu; Robert P. Coppes; Dianzheng Zhang; Yunlong Pan; Shegan Gao; Hao Zhang

doi:10.3390/cells11020277

Generation and Application of Inducible Chimeric RNA ASTN2-PAPPA(as) Knockin Mouse Model

Yichen Luo, Liang Du, Zhimeng Yao, Fan Liu, Kai Li, Feifei Li, Jianlin Zhu, Robert P. Coppes, Dianzheng Zhang, Yunlong Pan, Shegan Gao^*, Hao Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Chimeric RNAs (chiRNAs) play many previously unrecognized roles in different diseases including cancer. They can not only be used as biomarkers for diagnosis and prognosis of various diseases but also serve as potential therapeutic targets. In order to better understand the roles of chiRNAs in pathogenesis, we inserted human sequences into mouse genome and established a knockin mouse model of the tamoxifen-inducible expression of ASTN2-PAPPA antisense chimeric RNA (A-P(as)chiRNA). Mice carrying the A-P(as)chiRNA knockin gene do not display any apparent abnormalities in growth, fertility, histological, hematopoietic, and biochemical indices. Using this model, we dissected the role of A-P(as)chiRNA in chemical carcinogen 4-nitroquinoline 1-oxide (4NQO)-induced carcinogenesis of esophageal squamous cell carcinoma (ESCC). To our knowledge, we are the first to generate a chiRNA knockin mouse model using the Cre-loxP system. The model could be used to explore the roles of chiRNA in pathogenesis and potential targeted therapies.

Original language	English
Article number	277
Number of pages	10
Journal	Cells
Volume	11
Issue number	2
DOIs	https://doi.org/10.3390/cells11020277
Publication status	Published - 1-Jan-2022

Keywords

transcription-induced chimeric RNA
fusion RNA
A-P(as)chiRNA
genetically engineered mouse model
knockin mouse
cancer
PROGRESSION
PROTEIN

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@article{ce959871c15a45d5ac1ccc2137e0c29a,

title = "Generation and Application of Inducible Chimeric RNA ASTN2-PAPPA(as) Knockin Mouse Model",

abstract = "Chimeric RNAs (chiRNAs) play many previously unrecognized roles in different diseases including cancer. They can not only be used as biomarkers for diagnosis and prognosis of various diseases but also serve as potential therapeutic targets. In order to better understand the roles of chiRNAs in pathogenesis, we inserted human sequences into mouse genome and established a knockin mouse model of the tamoxifen-inducible expression of ASTN2-PAPPA antisense chimeric RNA (A-P(as)chiRNA). Mice carrying the A-P(as)chiRNA knockin gene do not display any apparent abnormalities in growth, fertility, histological, hematopoietic, and biochemical indices. Using this model, we dissected the role of A-P(as)chiRNA in chemical carcinogen 4-nitroquinoline 1-oxide (4NQO)-induced carcinogenesis of esophageal squamous cell carcinoma (ESCC). To our knowledge, we are the first to generate a chiRNA knockin mouse model using the Cre-loxP system. The model could be used to explore the roles of chiRNA in pathogenesis and potential targeted therapies.",

keywords = "transcription-induced chimeric RNA, fusion RNA, A-P(as)chiRNA, genetically engineered mouse model, knockin mouse, cancer, PROGRESSION, PROTEIN",

author = "Yichen Luo and Liang Du and Zhimeng Yao and Fan Liu and Kai Li and Feifei Li and Jianlin Zhu and Coppes, {Robert P.} and Dianzheng Zhang and Yunlong Pan and Shegan Gao and Hao Zhang",

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doi = "10.3390/cells11020277",

language = "English",

volume = "11",

journal = "Cells",

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TY - JOUR

T1 - Generation and Application of Inducible Chimeric RNA ASTN2-PAPPA(as) Knockin Mouse Model

AU - Luo, Yichen

AU - Du, Liang

AU - Yao, Zhimeng

AU - Liu, Fan

AU - Li, Kai

AU - Li, Feifei

AU - Zhu, Jianlin

AU - Coppes, Robert P.

AU - Zhang, Dianzheng

AU - Pan, Yunlong

AU - Gao, Shegan

AU - Zhang, Hao

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Chimeric RNAs (chiRNAs) play many previously unrecognized roles in different diseases including cancer. They can not only be used as biomarkers for diagnosis and prognosis of various diseases but also serve as potential therapeutic targets. In order to better understand the roles of chiRNAs in pathogenesis, we inserted human sequences into mouse genome and established a knockin mouse model of the tamoxifen-inducible expression of ASTN2-PAPPA antisense chimeric RNA (A-P(as)chiRNA). Mice carrying the A-P(as)chiRNA knockin gene do not display any apparent abnormalities in growth, fertility, histological, hematopoietic, and biochemical indices. Using this model, we dissected the role of A-P(as)chiRNA in chemical carcinogen 4-nitroquinoline 1-oxide (4NQO)-induced carcinogenesis of esophageal squamous cell carcinoma (ESCC). To our knowledge, we are the first to generate a chiRNA knockin mouse model using the Cre-loxP system. The model could be used to explore the roles of chiRNA in pathogenesis and potential targeted therapies.

AB - Chimeric RNAs (chiRNAs) play many previously unrecognized roles in different diseases including cancer. They can not only be used as biomarkers for diagnosis and prognosis of various diseases but also serve as potential therapeutic targets. In order to better understand the roles of chiRNAs in pathogenesis, we inserted human sequences into mouse genome and established a knockin mouse model of the tamoxifen-inducible expression of ASTN2-PAPPA antisense chimeric RNA (A-P(as)chiRNA). Mice carrying the A-P(as)chiRNA knockin gene do not display any apparent abnormalities in growth, fertility, histological, hematopoietic, and biochemical indices. Using this model, we dissected the role of A-P(as)chiRNA in chemical carcinogen 4-nitroquinoline 1-oxide (4NQO)-induced carcinogenesis of esophageal squamous cell carcinoma (ESCC). To our knowledge, we are the first to generate a chiRNA knockin mouse model using the Cre-loxP system. The model could be used to explore the roles of chiRNA in pathogenesis and potential targeted therapies.

KW - transcription-induced chimeric RNA

KW - fusion RNA

KW - A-P(as)chiRNA

KW - genetically engineered mouse model

KW - knockin mouse

KW - cancer

KW - PROGRESSION

KW - PROTEIN

U2 - 10.3390/cells11020277

DO - 10.3390/cells11020277

M3 - Article

SN - 2073-4409

VL - 11

JO - Cells

JF - Cells

IS - 2

M1 - 277

ER -

Generation and Application of Inducible Chimeric RNA ASTN2-PAPPA(as) Knockin Mouse Model

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Keywords

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