Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

Anika Nagelkerke; Johan Bussink; Fred C G J Sweep; Paul N Span

doi:10.1016/j.ab.2013.02.004

Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

Anika Nagelkerke^*, Johan Bussink, Fred C G J Sweep, Paul N Span

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

53 Citations (Scopus)

Abstract

The multicellular tumor spheroid model is a widely used culture system to mimic the three-dimensionality of tumors. Several methods and an even larger number of protocols are available to prepare spheroids from regular monolayers. This paper describes the assessment of several techniques frequently used to culture spheroids of breast cancer cells. We found that some methods produced suboptimal results. Ultimately, an adapted liquid overlay technique generated tight, compact and robust spheroids of the breast cancer cells tested.

Original language	English
Pages (from-to)	17-9
Number of pages	3
Journal	Analytical Biochemistry
Volume	437
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2013.02.004
Publication status	Published - 1-Jun-2013
Externally published	Yes

Keywords

Breast Neoplasms/pathology
Cell Adhesion
Cell Culture Techniques/methods
Cell Line, Tumor
Humans
Spheroids, Cellular/pathology

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KW - Cell Culture Techniques/methods

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