Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

Anika Nagelkerke*, Johan Bussink, Fred C G J Sweep, Paul N Span

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

53 Citations (Scopus)


The multicellular tumor spheroid model is a widely used culture system to mimic the three-dimensionality of tumors. Several methods and an even larger number of protocols are available to prepare spheroids from regular monolayers. This paper describes the assessment of several techniques frequently used to culture spheroids of breast cancer cells. We found that some methods produced suboptimal results. Ultimately, an adapted liquid overlay technique generated tight, compact and robust spheroids of the breast cancer cells tested.

Original languageEnglish
Pages (from-to)17-9
Number of pages3
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - 1-Jun-2013
Externally publishedYes


  • Breast Neoplasms/pathology
  • Cell Adhesion
  • Cell Culture Techniques/methods
  • Cell Line, Tumor
  • Humans
  • Spheroids, Cellular/pathology

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