Genetic and Molecular Characterization of a Cryptochrome from the Filamentous Fungus Neurospora crassa

Allan C. Froehlich, Chen-Hui Chen, William J. Belden, Cornelia Madeti, Till Roenneberg, Martha Merrow, Jennifer J. Loros, Jay C. Dunlap*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

49 Citations (Scopus)
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Abstract

In plants and animals, cryptochromes function as either photoreceptors or circadian clock components. We have examined the cryptochrome from the filamentous fungus Neurospora crassa and demonstrate that Neurospora cry encodes a DASH-type cryptochrome that appears capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). The cry transcript and CRY protein levels are strongly induced by blue light in a wc-1-dependent manner, and cry transcript is circadianly regulated, with a peak abundance opposite in phase to frq. Neither deletion nor overexpression of cry appears to perturb the free-running circadian clock. However, cry disruption knockout mutants show a small phase delay under circadian entrainment. Using electrophoretic mobility shift assays (EMSA), we show that CRY is capable of binding single- and double-stranded DNA (ssDNA and dsDNA, respectively) and ssRNA and dsRNA. Whole-genome microarray experiments failed to identify substantive transcriptional regulatory activity of cry under our laboratory conditions.

Original languageEnglish
Pages (from-to)738-750
Number of pages13
JournalEukaryotic Cell
Volume9
Issue number5
DOIs
Publication statusPublished - May-2010

Keywords

  • MAMMALIAN CIRCADIAN CLOCK
  • BLUE-LIGHT PHOTORECEPTORS
  • PHOTOLYASE ACTIVITY
  • HIGH-THROUGHPUT
  • DNA PHOTOLYASE
  • WHITE COLLAR-1
  • FEEDBACK LOOPS
  • ARABIDOPSIS
  • EXPRESSION
  • DROSOPHILA

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