Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)

L.A. Mesbah; T.J.A. Kneppers; F.L.W. Takken; P. Laurent; J. Hille; H.J.J. Nijkamp

doi:10.1007/s004380050940

Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)

L.A. Mesbah, T.J.A. Kneppers, F.L.W. Takken, P. Laurent, J. Hille, H.J.J. Nijkamp

Groningen Biomolecular Sciences and Biotechnology Institute

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Abstract

The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on chromosome 3 of tomato. We describe here the establishment of a 1383-kb YAC contig covering the Asc locus and a series of plants selected for recombination events around the Asc locus. It was shown that the YAC contig corresponds to a genetic distance of at least 11.2 cM. Thus, the recombination rate in the Asc region is six times higher (123 kb/cM) than the average for the tomato genome. Furthermore, the Asc locus could be localised to a 91-kb fragment, thus paving the way for the cloning and identification of the Asc gene(s) by complementation.

Original language	English
Number of pages	8
Journal	Molecular and General Genetics
Volume	261
Issue number	1
DOIs	https://doi.org/10.1007/s004380050940
Publication status	Published - 1998

Keywords

Recombination
Physical mapping
Map-based cloning
Alternaria stem canker
Tomato

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@article{7adcad525f2a4759a636857fc3c3eabf,

title = "Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)",

abstract = "The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on chromosome 3 of tomato. We describe here the establishment of a 1383-kb YAC contig covering the Asc locus and a series of plants selected for recombination events around the Asc locus. It was shown that the YAC contig corresponds to a genetic distance of at least 11.2 cM. Thus, the recombination rate in the Asc region is six times higher (123 kb/cM) than the average for the tomato genome. Furthermore, the Asc locus could be localised to a 91-kb fragment, thus paving the way for the cloning and identification of the Asc gene(s) by complementation.",

keywords = "Recombination, Physical mapping, Map-based cloning, Alternaria stem canker, Tomato",

author = "L.A. Mesbah and T.J.A. Kneppers and F.L.W. Takken and P. Laurent and J. Hille and H.J.J. Nijkamp",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1998",

doi = "10.1007/s004380050940",

language = "English",

volume = "261",

journal = "Molecular and General Genetics",

number = "1",

}

TY - JOUR

T1 - Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)

AU - Mesbah, L.A.

AU - Kneppers, T.J.A.

AU - Takken, F.L.W.

AU - Laurent, P.

AU - Hille, J.

AU - Nijkamp, H.J.J.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1998

Y1 - 1998

N2 - The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on chromosome 3 of tomato. We describe here the establishment of a 1383-kb YAC contig covering the Asc locus and a series of plants selected for recombination events around the Asc locus. It was shown that the YAC contig corresponds to a genetic distance of at least 11.2 cM. Thus, the recombination rate in the Asc region is six times higher (123 kb/cM) than the average for the tomato genome. Furthermore, the Asc locus could be localised to a 91-kb fragment, thus paving the way for the cloning and identification of the Asc gene(s) by complementation.

AB - The Alternaria stem canker disease of tomato is caused by the necrotrophic fungal pathogen Alternaria alternata f. sp. lycopersici (AAL). The fungus produces AAL toxins that kill the plant tissue. Resistance to the fungus segregates as a single locus, called Asc, and has been genetically mapped on chromosome 3 of tomato. We describe here the establishment of a 1383-kb YAC contig covering the Asc locus and a series of plants selected for recombination events around the Asc locus. It was shown that the YAC contig corresponds to a genetic distance of at least 11.2 cM. Thus, the recombination rate in the Asc region is six times higher (123 kb/cM) than the average for the tomato genome. Furthermore, the Asc locus could be localised to a 91-kb fragment, thus paving the way for the cloning and identification of the Asc gene(s) by complementation.

KW - Recombination

KW - Physical mapping

KW - Map-based cloning

KW - Alternaria stem canker

KW - Tomato

U2 - 10.1007/s004380050940

DO - 10.1007/s004380050940

M3 - Article

VL - 261

JO - Molecular and General Genetics

JF - Molecular and General Genetics

IS - 1

ER -

Genetic and physical analysis of a YAC contig spanning the fungal disease resistance locus Asc of tomato (Lycopersicon esculentum)

Abstract

Keywords

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