Genetically-modified bacteria and uses thereof

Jonathan Knight (Inventor), Cecilia Kvarnström Branneby (Inventor), Lubbert Dijkhuizen (Inventor), Mirjan Petrusma (Inventor), Laura Fernández de las Heras (Inventor)

Research output: Patent

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Abstract

A genetically-modified bacterium, for example of the class Actinobacteria, and the use of such a bacterium in the bioconversion of a steroidal substrate into a steroidal product of interest. A method of converting a steroidal substrate into a steroidal product of interest, wherein the method comprises: inoculating culture medium with genetically-modified bacteria according to any of Claims 1 to 28 and growing the bacterial culture until a target OD600 is reached; adding a steroidal substrate to the bacterial culture when the target OD600 is reached; culturing the bacterial culture so that the steroidal substrate is converted to the steroidal product of interest; and extracting and/or purifying the steroidal product of interest from the bacterial culture.
Original languageEnglish
Patent numberWO2020030799
Priority date09/08/2018
Filing date09/08/2019
Publication statusPublished - 13-Feb-2020

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