TY - JOUR
T1 - Genome-wide analysis reveals two novel mosaic regions containing an ACME with an identical DNA sequence in the MRSA ST398-t011 and MSSA ST8-t008 isolates
AU - Sabat, Artur J.
AU - Ilczyszyn, Weronika M.
AU - van Rijen, Miranda
AU - Akkerboom-Likhuta, Viktoria
AU - Sinha, Bhanu
AU - Kluytmans, Jan
AU - Miedzobrodzki, Jacek
AU - Grundmann, Hajo
AU - Friedrich, Alexander W.
N1 - © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: [email protected].
PY - 2015/5
Y1 - 2015/5
N2 - Objectives: The presence of the arginine catabolic mobile element (ACME) in Staphylococcus aureus has been reported to enhance the colonization of the human host. The aim of this study was to determine the genetic organization of composite islands harbouring ACME.Methods: Two ACME-positive S. aureus isolates obtained during two different surveys conducted in the Netherlands and Poland were characterized in this study. The isolates were analysed by spa typing, DNA micro-arrays and whole-genome sequencing.Results: The two isolates harboured a truncated yet fully functional ACME type II with an identical nucleotide sequence, but differed in their adjacent mobile genetic elements. The first strain was a livestock-associated ST398-t011 MRSA, which had a staphylococcal cassette chromosome mec (SCCmec) composite island composed of SCCpls adjacent to orfX followed by ACME type II and SCCmec type IVa. The second ACME-positive isolate was an ST8-t008 MSSA. Its composite island showed an SCC-like element carrying the ccrC gene followed by ACME II.Conclusions: This is the first report of an ACME in a livestock-associated MRSA ST398. It is also the first presentation of an ACME composite island structure in an MSSA isolate. Our findings indicate an extensive mosaicism of composite islands in S. aureus, which has implications for the transmissibility among humans and thus for public health.
AB - Objectives: The presence of the arginine catabolic mobile element (ACME) in Staphylococcus aureus has been reported to enhance the colonization of the human host. The aim of this study was to determine the genetic organization of composite islands harbouring ACME.Methods: Two ACME-positive S. aureus isolates obtained during two different surveys conducted in the Netherlands and Poland were characterized in this study. The isolates were analysed by spa typing, DNA micro-arrays and whole-genome sequencing.Results: The two isolates harboured a truncated yet fully functional ACME type II with an identical nucleotide sequence, but differed in their adjacent mobile genetic elements. The first strain was a livestock-associated ST398-t011 MRSA, which had a staphylococcal cassette chromosome mec (SCCmec) composite island composed of SCCpls adjacent to orfX followed by ACME type II and SCCmec type IVa. The second ACME-positive isolate was an ST8-t008 MSSA. Its composite island showed an SCC-like element carrying the ccrC gene followed by ACME II.Conclusions: This is the first report of an ACME in a livestock-associated MRSA ST398. It is also the first presentation of an ACME composite island structure in an MSSA isolate. Our findings indicate an extensive mosaicism of composite islands in S. aureus, which has implications for the transmissibility among humans and thus for public health.
KW - horizontal transfer
KW - whole-genome sequencing
KW - next-generation sequencing
KW - RESISTANT STAPHYLOCOCCUS-AUREUS
KW - CATABOLIC MOBILE ELEMENT
KW - CASSETTE CHROMOSOME MEC
KW - METHICILLIN-RESISTANT
KW - GENOTYPES
KW - BACTERIA
KW - VARIANT
KW - USA300
U2 - 10.1093/jac/dku531
DO - 10.1093/jac/dku531
M3 - Article
C2 - 25634990
SN - 0305-7453
VL - 70
SP - 1298
EP - 1302
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 5
ER -