Genomic characterization of coxsackievirus A22 from a regional university hospital in the Netherlands

Hayley Cassidy, Leonard Schuele, Hubert G. M. Niesters*, Coretta Van Leer-Buter, Erley Lizarazo-Forero

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Downloads (Pure)


Background: Enteroviruses are highly diverse with a wide spectrum of genotypes and clinical manifestations. Coxsackievirus A22 (CVA22) has been detected globally from sewage surveillance; however, currently there is limited information on its prevalence in patients, as well as available genomic data. Objective: We aimed to provide genomic and relative frequency data on CVA22 from a regional hospital perspective between 2013-2020. Study design: Sanger sequencing was performed on all samples with a positive enterovirus RT-qPCR result ( 3 weeks). Furthermore, we report the first two near-complete CVA22 sequences from Europe, which grouped with a strain previously isolated from Bangladesh in 1999. Conclusions: We show a highly diverse enterovirus genotype which causes infections annually, typically in autumn and winter, and is capable of recurrent infection in an immunocompromised patient. Furthermore, we highlight the use of NGS to complement conventional targeted Sanger sequencing.

Original languageEnglish
Article number105272
Number of pages5
JournalJournal of Clinical Virology
Publication statusPublished - Nov-2022


  • Enterovirus
  • Coxsackievirus A22
  • Next-generation sequencing
  • Prolonged infection

Cite this