Genomic DNA characterization of pork spleen by Raman spectroscopy

In this paper, the study of Raman signal enhancement due to interaction between ZnO rods and pork spleen DNA is reported. ZnO microstructures were synthesized by the Sol-Gel method and afterward combined with porcine spleen DNA extracted in the previous stages, following standardized cell lysis, deproteinization, and precipitation processes. Raman spectroscopy was used for the characterization of structures of ZnO and ZnO-DNA complex, and the results show the respective bands of ZnO wurtzite hexagonal phase for modes E-2 (M), A(1)(TO), E-2(High), E-1(LO), and 2LO. Due to the SERS effect in the spectral range from 200 to 1800 cm,(-1) Raman bands caused by vibrations of the deoxyribose C-O-C binding were also observed, producing deformation of the ring as shown in the 559 cm(-1) peak. The broad band at 782 cm(-1), together with the complex vibration of the string 5'-COPO-C3', is over a wide band of thymine (790 cm(-1)) or cytosine (780 cm(-1)). A prominent band near 1098 cm(-1) assigned to symmetric stretching vibration phosphodioxy group (PO2-) DNA backbone is most favoured in intensity by the addition of ZnO particles originated by the SERS effect. This effect suggests a possible mechanism for enhancing the Raman signal due to the electromagnetic interaction between a DNA molecule and the flat surface of the ZnO rod. (C) 2013 AIP Publishing LLC.