Genotype diversity of hepatitis C virus (HCV) in HCV-associated liver disease patients in Indonesia

Andi Utama, Navessa Padma Tania, Rama Dhenni, Rino Alvani Gani, Irsan Hasan, Andri Sanityoso, Syafruddin A. R. Lelosutan, Ruswhandi Martamala, Laurentius Adrianus Lesmana, Ali Sulaiman, Susan Tai

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Background: Hepatitis C virus (HCV) genotype distribution in Indonesia has been reported. However, the identification of HCV genotype was based on 5'-UTR or NS5B sequence. Aims: This study was aimed to observe HCV core sequence variation among HCV-associated liver disease patients in Jakarta, and to analyse the HCV genotype diversity based on the core sequence. Methods: Sixty-eight chronic hepatitis (CH), 48 liver cirrhosis (LC) and 34 hepatocellular carcinoma (HCC) were included in this study. HCV core variation was analysed by direct sequencing. Results: Alignment of HCV core sequences demonstrated that the core sequence was relatively varied among the genotype. Indeed, 237 bases of the core sequence could classify the HCV subtype; however, 236 bases failed to differentiate several subtypes. Based on 237 bases of the core sequences, the HCV strains were classified into genotypes 1 (subtypes 1a, 1b and 1c), 2 (subtypes 2a, 2e and 2f) and 3 (subtypes 3a and 3k). The HCV 1b (47.3%) was the most prevalent, followed by subtypes 1c (18.7%), 3k (10.7%), 2a (10.0%), 1a (6.7%), 2e (5.3%), 2f (0.7%) and 3a (0.7%). HCV 1b was the most common in all patients, and the prevalence increased with the severity of liver disease (36.8% in CH, 54.2% in LC and 58.8%in HCC). These results were similar to a previous report based on NS5B sequence analysis. Conclusion: Hepatitis C virus core sequence (237 bases) could identify the HCV subtype and the prevalence of HCV subtype based on core sequence was similar to those based on the NS5B region. © 2010 John Wiley & Sons A/S.
Original languageEnglish
Pages (from-to)1152-1160
Number of pages9
JournalLiver International
Issue number8
Publication statusPublished - 1-Sep-2010
Externally publishedYes


  • Core region
  • Genotype
  • Hepatitis C virus
  • Indonesia
  • Liver disease
  • alpha fetoprotein
  • adult
  • article
  • chronic hepatitis
  • disease severity
  • female
  • genotype
  • human
  • liver cell carcinoma
  • liver cirrhosis
  • major clinical study
  • male
  • nucleotide sequence
  • phylogeny
  • prevalence
  • sequence analysis
  • virus core
  • virus load
  • virus strain

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