Gentamicin release from polymethylmethacrylate bone cements and Staphylococcus aureus biofilm formation

H van de Belt, D Neut, W Schenk, [No Value] van Horn, HC van der Mei, HJ Busscher*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

102 Citations (Scopus)


We measured the formation of a Staphylococcus aureus biofilm in vitro on unloaded and gentamicin-loaded bone cements (CMW3 and Palaces R) and related the formation to antibiotic release rates. All experiments were done in triplicate. Microbial growth on gentamicin-loaded cements occurred despite the release of antibiotic. Biofilm formation an gentamicin loaded CMW3 bone cement was one fourth to one fifth less than on the unloaded bone cement, while biofilm formation an Palacos R bone cement was not significantly affected by antibiotic loading. More gentamicin was released from CMW3 (79 mg) than from Palacos R (70 mg), but the percentage gentamicin released after one week relative to the total amount incorporated was significantly lower for CMW3 (4.7%) than for Palaces R (8.4%), After one day, subinhibitory concentrations of antibiotics were eluted from the cements. We concluded that antibiotic-loaded bone cement does not necessarily inhibit the formation of an infectious biofilm in vitro.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
Issue number6
Publication statusPublished - Dec-2000


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