GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis

R Larsen; TG Kloosterman; J Kok; OP Kuipers

doi:10.1128/JB.00025-06

GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis

R Larsen, TG Kloosterman, J Kok, OP Kuipers^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

33 Citations (Scopus)

210 Downloads (Pure)

Abstract

We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TN ACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.

Original language	English
Pages (from-to)	4978-4982
Number of pages	5
Journal	Journal of Bacteriology
Volume	188
Issue number	13
DOIs	https://doi.org/10.1128/JB.00025-06
Publication status	Published - Jul-2006

Keywords

AMINO-ACID BIOSYNTHESIS
BACILLUS-SUBTILIS
STREPTOCOCCUS-LACTIS
TNRA
GENE
PROMOTER
CODY
IDENTIFICATION
EXPRESSION
OPERON

Access to Document

10.1128/JB.00025-06

2006JBacteriolLarsen.pdfFinal publisher's version, 316 KB

Handle.net

Persistent link

Cite this

@article{427e7d2ff7e1496687397963a671728a,

title = "GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis",

abstract = "We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TN ACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.",

keywords = "AMINO-ACID BIOSYNTHESIS, BACILLUS-SUBTILIS, STREPTOCOCCUS-LACTIS, TNRA, GENE, PROMOTER, CODY, IDENTIFICATION, EXPRESSION, OPERON",

author = "R Larsen and TG Kloosterman and J Kok and OP Kuipers",

year = "2006",

month = jul,

doi = "10.1128/JB.00025-06",

language = "English",

volume = "188",

pages = "4978--4982",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "AMER SOC MICROBIOLOGY",

number = "13",

}

TY - JOUR

T1 - GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis

AU - Larsen, R

AU - Kloosterman, TG

AU - Kok, J

AU - Kuipers, OP

PY - 2006/7

Y1 - 2006/7

N2 - We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TN ACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.

AB - We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TN ACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.

KW - AMINO-ACID BIOSYNTHESIS

KW - BACILLUS-SUBTILIS

KW - STREPTOCOCCUS-LACTIS

KW - TNRA

KW - GENE

KW - PROMOTER

KW - CODY

KW - IDENTIFICATION

KW - EXPRESSION

KW - OPERON

U2 - 10.1128/JB.00025-06

DO - 10.1128/JB.00025-06

M3 - Article

VL - 188

SP - 4978

EP - 4982

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 13

ER -