Glomerular immunoglobulin deposits induce glomerular inflammation in pregnant but not in non-pregnant rats

MM Faas*, G Van Der Schaaf, M Schipper, H Moes

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

PROBLEM: Does an inflammatory stimulus evoke a more intense inflammatory response in pregnant rats as compared with nonpregnant rats?

METHOD OF STUDY: Non-pregnant rats were injected with antibodies against the glomerular basement membrane (GBM), 14 days before pregnancy, to induce a subclinical glomerulonephritis. Part of the rats were rendered pregnant, the others remained nonpregnant throughout the experiment. Two experiments were performed: in experiment 1, pregnant and non-pregnant rats were killed at various intervals after the injection with antibody and parameters characteristic of a glomerular inflammation were evaluated using immunohistology on cryostat kidney sections and liver sections. In experiment 2, 24-hr urinary protein excretion was measured at various days after the injection in pregnant and non-pregnant rats.

RESULTS: Experiment 1 revealed that a significant glomerular inflammation, as characterized by increased numbers of monocytes and LFA-1 positive cells per glomerulus, was only observed in pregnant rats with glomerulonephritis. Experiment 2 revealed that only pregnant rats with glomerulonephritis showed increased urinary protein excretion.

CONCLUSION: The fact that glomerular inflammation coincides with proteinuria only in pregnant rats with glomerulonephritis, may suggest that these phenomena are causally related and promoted by the pregnant condition.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume49
Issue number1
Publication statusPublished - Jan-2003

Keywords

  • pregnancy
  • inflammation
  • monocytes
  • proteinuria
  • LOW-DOSE-ENDOTOXIN
  • LEUKOCYTE ADHESION MOLECULE-1
  • EXPERIMENTAL PREECLAMPSIA
  • EXPRESSION
  • GLOMERULONEPHRITIS
  • PROTEINURIA
  • INFUSION

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