Guanine quadruplex structures localize to heterochromatin

Roland F. Hoffmann; Yuri M. Moshkin; Stijn Mouton; Nicola A. Grzeschik; Ruby D. Kalicharan; Jeroen Kuipers; Anouk H. G. Wolters; Kazuki Nishida; Aleksander V. Romashchenko; Jan Postberg; Hans Lipps; Eugene Berezikov; Ody C. M. Sibon; Ben N. G. Giepmans; Peter M. Lansdorp

doi:10.1093/nar/gkv900

Guanine quadruplex structures localize to heterochromatin

Roland F. Hoffmann, Yuri M. Moshkin, Stijn Mouton, Nicola A. Grzeschik, Ruby D. Kalicharan, Jeroen Kuipers, Anouk H. G. Wolters, Kazuki Nishida, Aleksander V. Romashchenko, Jan Postberg, Hans Lipps, Eugene Berezikov, Ody C. M. Sibon, Ben N. G. Giepmans, Peter M. Lansdorp^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.

Original language	English
Pages (from-to)	152-163
Number of pages	12
Journal	Nucleic Acids Research
Volume	44
Issue number	1
Early online date	17-Sept-2015
DOIs	https://doi.org/10.1093/nar/gkv900
Publication status	Published - 8-Jan-2016

Keywords

MELANOGASTER POLYTENE CHROMOSOMES
SIZED DNA-MOLECULES
DROSOPHILA-MELANOGASTER
STEM-CELLS
DEPENDENT MANNER
X-SYNDROME
RICH DNA
G4 DNA
CHROMATIN
PROTEIN

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Guanine quadruplex structures localize to heterochromatinFinal publisher's version, 16.1 MBLicence: CC BY-NC

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Hoffmann, R. F., Moshkin, Y. M., Mouton, S., Grzeschik, N. A., Kalicharan, R. D., Kuipers, J., Wolters, A. H. G., Nishida, K., Romashchenko, A. V., Postberg, J., Lipps, H., Berezikov, E., Sibon, O. C. M., Giepmans, B. N. G., & Lansdorp, P. M. (2016). Guanine quadruplex structures localize to heterochromatin. Nucleic Acids Research, 44(1), 152-163. https://doi.org/10.1093/nar/gkv900

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title = "Guanine quadruplex structures localize to heterochromatin",

abstract = "Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.",

keywords = "MELANOGASTER POLYTENE CHROMOSOMES, SIZED DNA-MOLECULES, DROSOPHILA-MELANOGASTER, STEM-CELLS, DEPENDENT MANNER, X-SYNDROME, RICH DNA, G4 DNA, CHROMATIN, PROTEIN",

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AU - Hoffmann, Roland F.

AU - Moshkin, Yuri M.

AU - Mouton, Stijn

AU - Grzeschik, Nicola A.

AU - Kalicharan, Ruby D.

AU - Kuipers, Jeroen

AU - Wolters, Anouk H. G.

AU - Nishida, Kazuki

AU - Romashchenko, Aleksander V.

AU - Postberg, Jan

AU - Lipps, Hans

AU - Berezikov, Eugene

AU - Sibon, Ody C. M.

AU - Giepmans, Ben N. G.

AU - Lansdorp, Peter M.

N1 - © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2016/1/8

Y1 - 2016/1/8

N2 - Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.

AB - Increasing amounts of data support a role for guanine quadruplex (G4) DNA and RNA structures in various cellular processes. We stained different organisms with monoclonal antibody 1H6 specific for G4 DNA. Strikingly, immuno-electron microscopy showed exquisite specificity for heterochromatin. Polytene chromosomes from Drosophila salivary glands showed bands that co-localized with heterochromatin proteins HP1 and the SNF2 domain-containing protein SUUR. Staining was retained in SUUR knock-out mutants but lost upon overexpression of SUUR. Somatic cells in Macrostomum lignano were strongly labeled, but pluripotent stem cells labeled weakly. Similarly, germline stem cells in Drosophila ovaries were weakly labeled compared to most other cells. The unexpected presence of G4 structures in heterochromatin and the difference in G4 staining between somatic cells and stem cells with germline DNA in ciliates, flatworms, flies and mammals point to a conserved role for G4 structures in nuclear organization and cellular differentiation.

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KW - DROSOPHILA-MELANOGASTER

KW - STEM-CELLS

KW - DEPENDENT MANNER

KW - X-SYNDROME

KW - RICH DNA

KW - G4 DNA

KW - CHROMATIN

KW - PROTEIN

U2 - 10.1093/nar/gkv900

DO - 10.1093/nar/gkv900

M3 - Article

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SN - 0305-1048

VL - 44

SP - 152

EP - 163

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 1

ER -

Guanine quadruplex structures localize to heterochromatin

Abstract

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