Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky; Kotb Abdelmohsen; Akihisa Abe; Md Joynal Abedin; Hagai Abeliovich; Abraham Acevedo Arozena; Hiroaki Adachi; Christopher M. Adams; Peter D. Adams; Khosrow Adeli; Peter J. Adhihetty; Sharon G. Adler; Galila Agam; Rajesh Agarwal; Manish K. Aghi; Maria Agnello; Patrizia Agostinis; Patricia V. Aguilar; Julio Aguirre-Ghiso; Edoardo M. Airoldi; Slimane Ait-Si-Ali; Takahiko Akematsu; Emmanuel T. Akporiaye; Mohamed Al-Rubeai; Guillermo M. Albaiceta; Chris Albanese; Diego Albani; Matthew L. Albert; Jesus Aldudo; Hana Alguel; Mehrdad Alirezaei; Iraide Alloza; Alexandru Almasan; Maylin Almonte-Beceril; Emad S. Alnemri; Covadonga Alonso; Nihal Altan-Bonnet; Dario C. Altieri; Silvia Alvarez; Lydia Alvarez-Erviti; Sandro Alves; Giuseppina Amadoro; Atsuo Amano; Consuelo Amantini; Santiago Ambrosio; Ivano Amelio; Amal O. Amer; Mohamed Amessou; Angelika Amon; Fulvio Reggiori; Rubén Gómez-Sánchez

doi:10.1080/15548627.2015.1100356

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky^*, Kotb Abdelmohsen, Akihisa Abe, Md Joynal Abedin, Hagai Abeliovich, Abraham Acevedo Arozena, Hiroaki Adachi, Christopher M. Adams, Peter D. Adams, Khosrow Adeli, Peter J. Adhihetty, Sharon G. Adler, Galila Agam, Rajesh Agarwal, Manish K. Aghi, Maria Agnello, Patrizia Agostinis, Patricia V. Aguilar, Julio Aguirre-Ghiso, Edoardo M. AiroldiSlimane Ait-Si-Ali, Takahiko Akematsu, Emmanuel T. Akporiaye, Mohamed Al-Rubeai, Guillermo M. Albaiceta, Chris Albanese, Diego Albani, Matthew L. Albert, Jesus Aldudo, Hana Alguel, Mehrdad Alirezaei, Iraide Alloza, Alexandru Almasan, Maylin Almonte-Beceril, Emad S. Alnemri, Covadonga Alonso, Nihal Altan-Bonnet, Dario C. Altieri, Silvia Alvarez, Lydia Alvarez-Erviti, Sandro Alves, Giuseppina Amadoro, Atsuo Amano, Consuelo Amantini, Santiago Ambrosio, Ivano Amelio, Amal O. Amer, Mohamed Amessou, Angelika Amon, Fulvio Reggiori, Rubén Gómez-Sánchez

^*Corresponding author for this work

Microbes in Health and Disease (MHD)

Research output: Contribution to journal › Review article › peer-review

4474 Citations (Scopus)

Original language	English
Pages (from-to)	1-222
Number of pages	222
Journal	Autophagy
Volume	12
Issue number	1
DOIs	https://doi.org/10.1080/15548627.2015.1100356
Publication status	Published - 2016

Keywords

autolysosome
autophagosome
chaperone-mediated autophagy
flux
LC3
lysosome
macroautophagy
phagophore
stress
vacuole
CHAPERONE-MEDIATED AUTOPHAGY
ACTIVATED PROTEIN-KINASE
PROGRAMMED CELL-DEATH
STARVATION-INDUCED AUTOPHAGY
ENDOPLASMIC-RETICULUM STRESS
NF-KAPPA-B
VACUOLE TARGETING PATHWAY
ISOLATED RAT HEPATOCYTES
YEAST SACCHAROMYCES-CEREVISIAE
GLUCAGON-INDUCED AUTOPHAGY

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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
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Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., Adhihetty, P. J., Adler, S. G., Agam, G., Agarwal, R., Aghi, M. K., Agnello, M., Agostinis, P., Aguilar, P. V., Aguirre-Ghiso, J., ... Gómez-Sánchez, R. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222. https://doi.org/10.1080/15548627.2015.1100356

@article{b339ea1e000445a2ae7d56d177cb9709,

title = "Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)",

keywords = "autolysosome, autophagosome, chaperone-mediated autophagy, flux, LC3, lysosome, macroautophagy, phagophore, stress, vacuole, CHAPERONE-MEDIATED AUTOPHAGY, ACTIVATED PROTEIN-KINASE, PROGRAMMED CELL-DEATH, STARVATION-INDUCED AUTOPHAGY, ENDOPLASMIC-RETICULUM STRESS, NF-KAPPA-B, VACUOLE TARGETING PATHWAY, ISOLATED RAT HEPATOCYTES, YEAST SACCHAROMYCES-CEREVISIAE, GLUCAGON-INDUCED AUTOPHAGY",

author = "Klionsky, {Daniel J.} and Kotb Abdelmohsen and Akihisa Abe and Abedin, {Md Joynal} and Hagai Abeliovich and Arozena, {Abraham Acevedo} and Hiroaki Adachi and Adams, {Christopher M.} and Adams, {Peter D.} and Khosrow Adeli and Adhihetty, {Peter J.} and Adler, {Sharon G.} and Galila Agam and Rajesh Agarwal and Aghi, {Manish K.} and Maria Agnello and Patrizia Agostinis and Aguilar, {Patricia V.} and Julio Aguirre-Ghiso and Airoldi, {Edoardo M.} and Slimane Ait-Si-Ali and Takahiko Akematsu and Akporiaye, {Emmanuel T.} and Mohamed Al-Rubeai and Albaiceta, {Guillermo M.} and Chris Albanese and Diego Albani and Albert, {Matthew L.} and Jesus Aldudo and Hana Alguel and Mehrdad Alirezaei and Iraide Alloza and Alexandru Almasan and Maylin Almonte-Beceril and Alnemri, {Emad S.} and Covadonga Alonso and Nihal Altan-Bonnet and Altieri, {Dario C.} and Silvia Alvarez and Lydia Alvarez-Erviti and Sandro Alves and Giuseppina Amadoro and Atsuo Amano and Consuelo Amantini and Santiago Ambrosio and Ivano Amelio and Amer, {Amal O.} and Mohamed Amessou and Angelika Amon and Fulvio Reggiori and Rub{\'e}n G{\'o}mez-S{\'a}nchez",

year = "2016",

doi = "10.1080/15548627.2015.1100356",

language = "English",

volume = "12",

pages = "1--222",

journal = "Autophagy",

issn = "1554-8627",

publisher = "Taylor & Francis Group",

number = "1",

}

Klionsky, DJ, Abdelmohsen, K, Abe, A, Abedin, MJ, Abeliovich, H, Arozena, AA, Adachi, H, Adams, CM, Adams, PD, Adeli, K, Adhihetty, PJ, Adler, SG, Agam, G, Agarwal, R, Aghi, MK, Agnello, M, Agostinis, P, Aguilar, PV, Aguirre-Ghiso, J, Airoldi, EM, Ait-Si-Ali, S, Akematsu, T, Akporiaye, ET, Al-Rubeai, M, Albaiceta, GM, Albanese, C, Albani, D, Albert, ML, Aldudo, J, Alguel, H, Alirezaei, M, Alloza, I, Almasan, A, Almonte-Beceril, M, Alnemri, ES, Alonso, C, Altan-Bonnet, N, Altieri, DC, Alvarez, S, Alvarez-Erviti, L, Alves, S, Amadoro, G, Amano, A, Amantini, C, Ambrosio, S, Amelio, I, Amer, AO, Amessou, M, Amon, A, Reggiori, F & Gómez-Sánchez, R 2016, 'Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)', Autophagy, vol. 12, no. 1, pp. 1-222. https://doi.org/10.1080/15548627.2015.1100356

TY - JOUR

T1 - Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

AU - Klionsky, Daniel J.

AU - Abdelmohsen, Kotb

AU - Abe, Akihisa

AU - Abedin, Md Joynal

AU - Abeliovich, Hagai

AU - Arozena, Abraham Acevedo

AU - Adachi, Hiroaki

AU - Adams, Christopher M.

AU - Adams, Peter D.

AU - Adeli, Khosrow

AU - Adhihetty, Peter J.

AU - Adler, Sharon G.

AU - Agam, Galila

AU - Agarwal, Rajesh

AU - Aghi, Manish K.

AU - Agnello, Maria

AU - Agostinis, Patrizia

AU - Aguilar, Patricia V.

AU - Aguirre-Ghiso, Julio

AU - Airoldi, Edoardo M.

AU - Ait-Si-Ali, Slimane

AU - Akematsu, Takahiko

AU - Akporiaye, Emmanuel T.

AU - Al-Rubeai, Mohamed

AU - Albaiceta, Guillermo M.

AU - Albanese, Chris

AU - Albani, Diego

AU - Albert, Matthew L.

AU - Aldudo, Jesus

AU - Alguel, Hana

AU - Alirezaei, Mehrdad

AU - Alloza, Iraide

AU - Almasan, Alexandru

AU - Almonte-Beceril, Maylin

AU - Alnemri, Emad S.

AU - Alonso, Covadonga

AU - Altan-Bonnet, Nihal

AU - Altieri, Dario C.

AU - Alvarez, Silvia

AU - Alvarez-Erviti, Lydia

AU - Alves, Sandro

AU - Amadoro, Giuseppina

AU - Amano, Atsuo

AU - Amantini, Consuelo

AU - Ambrosio, Santiago

AU - Amelio, Ivano

AU - Amer, Amal O.

AU - Amessou, Mohamed

AU - Amon, Angelika

AU - Reggiori, Fulvio

AU - Gómez-Sánchez, Rubén

PY - 2016

Y1 - 2016

KW - autolysosome

KW - autophagosome

KW - chaperone-mediated autophagy

KW - flux

KW - LC3

KW - lysosome

KW - macroautophagy

KW - phagophore

KW - stress

KW - vacuole

KW - CHAPERONE-MEDIATED AUTOPHAGY

KW - ACTIVATED PROTEIN-KINASE

KW - PROGRAMMED CELL-DEATH

KW - STARVATION-INDUCED AUTOPHAGY

KW - ENDOPLASMIC-RETICULUM STRESS

KW - NF-KAPPA-B

KW - VACUOLE TARGETING PATHWAY

KW - ISOLATED RAT HEPATOCYTES

KW - YEAST SACCHAROMYCES-CEREVISIAE

KW - GLUCAGON-INDUCED AUTOPHAGY

U2 - 10.1080/15548627.2015.1100356

DO - 10.1080/15548627.2015.1100356

M3 - Review article

SN - 1554-8627

VL - 12

SP - 1

EP - 222

JO - Autophagy

JF - Autophagy

IS - 1

ER -