H-1, C-13, and N-15 resonance assignment of photoactive yellow protein

Trijntje J. Pool; Nur Alia Oktaviani; Hironari Kamikubo; Mikio Kataoka; Frans A. A. Mulder

doi:10.1007/s12104-012-9387-9

H-1, C-13, and N-15 resonance assignment of photoactive yellow protein

Trijntje J. Pool
, Nur Alia Oktaviani
, Hironari Kamikubo
, Mikio Kataoka
, Frans A. A. Mulder^*

^*Corresponding author for this work

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Photoactive yellow protein (PYP) is involved in the negative phototactic response towards blue light of the bacterium Halorhodospira halophila. Here, we report nearly complete backbone and side chain H-1, C-13 and N-15 resonance assignments at pH 5.8 and 20 A degrees C of PYP in its electronic ground state.

Original language	English
Pages (from-to)	97-100
Number of pages	4
Journal	Biomolecular nmr assignments
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s12104-012-9387-9
Publication status	Published - Apr-2013

Keywords

PYP
Halorhodospira halophila
para-coumaric acid
NMR spectroscopy
Photoactivation
ISOTOPICALLY-ENRICHED PROTEINS
2-DIMENSIONAL NMR EXPERIMENTS
ECTOTHIORHODOSPIRA-HALOPHILA
C-13/N-15-ENRICHED PROTEINS
C-13-LABELED PROTEINS
HIGH-SENSITIVITY
HYDROGEN-BOND
ACTIVE-SITE
SIDE-CHAINS
SPECTROSCOPY

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H-1, C-13, and N-15 resonance assignment of photoactive yellow proteinFinal publisher's version, 326 KBLicence: CC BY

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@article{7376d89df32f43f4a44d6c81f4a858fa,

title = "H-1, C-13, and N-15 resonance assignment of photoactive yellow protein",

abstract = "Photoactive yellow protein (PYP) is involved in the negative phototactic response towards blue light of the bacterium Halorhodospira halophila. Here, we report nearly complete backbone and side chain H-1, C-13 and N-15 resonance assignments at pH 5.8 and 20 A degrees C of PYP in its electronic ground state.",

keywords = "PYP, Halorhodospira halophila, para-coumaric acid, NMR spectroscopy, Photoactivation, ISOTOPICALLY-ENRICHED PROTEINS, 2-DIMENSIONAL NMR EXPERIMENTS, ECTOTHIORHODOSPIRA-HALOPHILA, C-13/N-15-ENRICHED PROTEINS, C-13-LABELED PROTEINS, HIGH-SENSITIVITY, HYDROGEN-BOND, ACTIVE-SITE, SIDE-CHAINS, SPECTROSCOPY",

author = "Pool, \{Trijntje J.\} and Oktaviani, \{Nur Alia\} and Hironari Kamikubo and Mikio Kataoka and Mulder, \{Frans A. A.\}",

year = "2013",

month = apr,

doi = "10.1007/s12104-012-9387-9",

language = "English",

volume = "7",

pages = "97--100",

journal = "Biomolecular nmr assignments",

issn = "1874-2718",

number = "1",

}

TY - JOUR

T1 - H-1, C-13, and N-15 resonance assignment of photoactive yellow protein

AU - Pool, Trijntje J.

AU - Oktaviani, Nur Alia

AU - Kamikubo, Hironari

AU - Kataoka, Mikio

AU - Mulder, Frans A. A.

PY - 2013/4

Y1 - 2013/4

N2 - Photoactive yellow protein (PYP) is involved in the negative phototactic response towards blue light of the bacterium Halorhodospira halophila. Here, we report nearly complete backbone and side chain H-1, C-13 and N-15 resonance assignments at pH 5.8 and 20 A degrees C of PYP in its electronic ground state.

AB - Photoactive yellow protein (PYP) is involved in the negative phototactic response towards blue light of the bacterium Halorhodospira halophila. Here, we report nearly complete backbone and side chain H-1, C-13 and N-15 resonance assignments at pH 5.8 and 20 A degrees C of PYP in its electronic ground state.

KW - PYP

KW - Halorhodospira halophila

KW - para-coumaric acid

KW - NMR spectroscopy

KW - Photoactivation

KW - ISOTOPICALLY-ENRICHED PROTEINS

KW - 2-DIMENSIONAL NMR EXPERIMENTS

KW - ECTOTHIORHODOSPIRA-HALOPHILA

KW - C-13/N-15-ENRICHED PROTEINS

KW - C-13-LABELED PROTEINS

KW - HIGH-SENSITIVITY

KW - HYDROGEN-BOND

KW - ACTIVE-SITE

KW - SIDE-CHAINS

KW - SPECTROSCOPY

U2 - 10.1007/s12104-012-9387-9

DO - 10.1007/s12104-012-9387-9

M3 - Article

SN - 1874-2718

VL - 7

SP - 97

EP - 100

JO - Biomolecular nmr assignments

JF - Biomolecular nmr assignments

IS - 1

ER -

H-1, C-13, and N-15 resonance assignment of photoactive yellow protein

Abstract

Keywords

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