H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5

Sander S. van Leeuwen; Bas J. H. Kuipers; Lubbert Dijkhuizen; Johannis P. Kamerling

doi:10.1016/j.carres.2014.08.012

H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5

Sander S. van Leeuwen, Bas J. H. Kuipers, Lubbert Dijkhuizen^*, Johannis P. Kamerling

^*Corresponding author for this work

Host-Microbe Interactions

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Abstract

Vivinal (R) GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans beta-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal (R) GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag+ column) and H-1 NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D H-1/C-13 NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99% of the product, have been characterized. Detailed H-1 and C-13 NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included. (C) 2014 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	59-73
Number of pages	15
Journal	Carbohydrate Research
Volume	400
DOIs	https://doi.org/10.1016/j.carres.2014.08.012
Publication status	Published - 5-Dec-2014

Keywords

Galacto-oligosaccharides
NMR spectroscopy
Vivinal (R) GOS
Bacillus circulans beta-galactosidase
Prebiotics
BETA-GALACTOSIDASE
BACILLUS-CIRCULANS
STATE

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H-1 NMR analysis of the lactose beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5
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Corrigendum to “1H NMR analysis of the lactose/β-galactosidase-derived galacto-oligosaccharide components of Vivinal® GOS up to DP5” [Carbohydr. Res. 400 (2014) 59–73]
van Leeuwen, S. S., Kuipers, B. J. H., Dijkhuizen, L. & Kamerling, J. P., Jan-2016, In: Carbohydrate Research. 419, p. 69-70 2 p.
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title = "H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5",

abstract = "Vivinal (R) GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans beta-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal (R) GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag+ column) and H-1 NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D H-1/C-13 NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99\% of the product, have been characterized. Detailed H-1 and C-13 NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included. (C) 2014 Elsevier Ltd. All rights reserved.",

keywords = "Galacto-oligosaccharides, NMR spectroscopy, Vivinal (R) GOS, Bacillus circulans beta-galactosidase, Prebiotics, BETA-GALACTOSIDASE, BACILLUS-CIRCULANS, STATE",

author = "\{van Leeuwen\}, \{Sander S.\} and Kuipers, \{Bas J. H.\} and Lubbert Dijkhuizen and Kamerling, \{Johannis P.\}",

year = "2014",

month = dec,

day = "5",

doi = "10.1016/j.carres.2014.08.012",

language = "English",

volume = "400",

pages = "59--73",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "ELSEVIER SCI LTD",

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T1 - H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5

AU - van Leeuwen, Sander S.

AU - Kuipers, Bas J. H.

AU - Dijkhuizen, Lubbert

AU - Kamerling, Johannis P.

PY - 2014/12/5

Y1 - 2014/12/5

N2 - Vivinal (R) GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans beta-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal (R) GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag+ column) and H-1 NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D H-1/C-13 NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99% of the product, have been characterized. Detailed H-1 and C-13 NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included. (C) 2014 Elsevier Ltd. All rights reserved.

AB - Vivinal (R) GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans beta-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal (R) GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag+ column) and H-1 NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D H-1/C-13 NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99% of the product, have been characterized. Detailed H-1 and C-13 NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included. (C) 2014 Elsevier Ltd. All rights reserved.

KW - Galacto-oligosaccharides

KW - NMR spectroscopy

KW - Vivinal (R) GOS

KW - Bacillus circulans beta-galactosidase

KW - Prebiotics

KW - BETA-GALACTOSIDASE

KW - BACILLUS-CIRCULANS

KW - STATE

U2 - 10.1016/j.carres.2014.08.012

DO - 10.1016/j.carres.2014.08.012

M3 - Article

SN - 0008-6215

VL - 400

SP - 59

EP - 73

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5

Abstract

Keywords

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Research output

Corrigendum to “1H NMR analysis of the lactose/β-galactosidase-derived galacto-oligosaccharide components of Vivinal® GOS up to DP5” [Carbohydr. Res. 400 (2014) 59–73]

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