H-1 NMR analysis of the lactose/beta-galactosidase-derived galacto-oligosaccharide components of Vivinal (R) GOS up to DP5

Sander S. van Leeuwen, Bas J. H. Kuipers, Lubbert Dijkhuizen*, Johannis P. Kamerling

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

58 Citations (Scopus)

Abstract

Vivinal (R) GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans beta-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal (R) GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag+ column) and H-1 NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D H-1/C-13 NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99% of the product, have been characterized. Detailed H-1 and C-13 NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included. (C) 2014 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)59-73
Number of pages15
JournalCarbohydrate Research
Volume400
DOIs
Publication statusPublished - 5-Dec-2014

Keywords

  • Galacto-oligosaccharides
  • NMR spectroscopy
  • Vivinal (R) GOS
  • Bacillus circulans beta-galactosidase
  • Prebiotics
  • BETA-GALACTOSIDASE
  • BACILLUS-CIRCULANS
  • STATE

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