Helicases FANCJ, RTEL1 and BLM Act on Guanine Quadruplex DNA in Vivo

Peter Lansdorp*, Niek van Wietmarschen

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)
31 Downloads (Pure)

Abstract

Guanine quadruplex (G4) structures are among the most stable secondary DNA structures that can form in vitro, and evidence for their existence in vivo has been steadily accumulating. Originally described mainly for their deleterious effects on genome stability, more recent research has focused on (potential) functions of G4 structures in telomere maintenance, gene expression, and other cellular processes. The combined research on G4 structures has revealed that properly regulating G4 DNA structures in cells is important to prevent genome instability and disruption of normal cell function. In this short review we provide some background and historical context of our work resulting in the identification of FANCJ, RTEL1 and BLM as helicases that act on G4 structures in vivo. Taken together these studies highlight important roles of different G4 DNA structures and specific G4 helicases at selected genomic locations and telomeres in regulating gene expression and maintaining genome stability.

Original languageEnglish
Article number870
Number of pages16
JournalGenes
Volume10
Issue number11
DOIs
Publication statusPublished - Nov-2019

Keywords

  • Guanine quadruplex (G4) DNA structures
  • G4 helicases
  • DOG-1
  • FANCJ
  • RTEL1
  • BLM
  • sister chromatid exchange events (SCEs)
  • genomic mapping of SCEs
  • molecular phenotype
  • single cell Strand-seq
  • BLOOMS-SYNDROME HELICASE
  • SYNDROME GENE-PRODUCT
  • TELOMERE LENGTH
  • GENOME INSTABILITY
  • STRAND SYNTHESIS
  • BINDING PROTEIN
  • STEM-CELLS
  • REPLICATION
  • PROMOTES
  • ANEMIA

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