TY - JOUR
T1 - High peroxidase activity in cell cultures of artemisia annua with minute artemisinin contents
AU - Woerdenbag, Herman J.
AU - Pras, Niesko
AU - Uden, Wim
AU - Boer, Arno
AU - Batterman, Sieb
AU - Visser, Jan F.
AU - Malingré, Theo M.
PY - 1992/9
Y1 - 1992/9
N2 - In callus and cell suspension cultures oí Artemisia annua minute amounts of artemisinin were found. In these cultures, a high peroxidase activity was measured, intracellularly and, especially, in the medium. In vitro, artemisinin rapidly decomposed when incubated with a cell homogenate of A. annua or with spent culture medium, as well as in a solution of commercially obtained horseradish peroxidase (EC 1.11.1.7). In contrast, the peroxidase activity in the intact plant, containing considerable amounts of artemisinin, was low. We suggest that the high peroxidase activity in A. annua cell cultures contributes to their very low artemisinin contents.
AB - In callus and cell suspension cultures oí Artemisia annua minute amounts of artemisinin were found. In these cultures, a high peroxidase activity was measured, intracellularly and, especially, in the medium. In vitro, artemisinin rapidly decomposed when incubated with a cell homogenate of A. annua or with spent culture medium, as well as in a solution of commercially obtained horseradish peroxidase (EC 1.11.1.7). In contrast, the peroxidase activity in the intact plant, containing considerable amounts of artemisinin, was low. We suggest that the high peroxidase activity in A. annua cell cultures contributes to their very low artemisinin contents.
UR - http://www.scopus.com/inward/record.url?scp=84963203640&partnerID=8YFLogxK
U2 - 10.1080/10575639208048899
DO - 10.1080/10575639208048899
M3 - Article
AN - SCOPUS:84963203640
SN - 1057-5634
VL - 1
SP - 121
EP - 128
JO - Natural Product Letters
JF - Natural Product Letters
IS - 2
ER -