Highly Parallel Transport Recordings on a Membrane-on-Nanopore Chip at Single Molecule Resolution

Michael Urban, Alexander Kleefen, Nobina Mukherjee, Patrick Seelheim, Barbara Windschiegl, Marc Vor Der Brueggen, Armagan Kocer, Robert Tampe*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250 000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.

Original languageEnglish
Pages (from-to)1674-1680
Number of pages7
JournalNano Letters
Volume14
Issue number3
Publication statusPublished - 13-Feb-2014

Keywords

  • Biosensor
  • membrane proteins
  • nanopore
  • nanofabrication
  • lab-on-chip
  • suspended lipid bilayer
  • MECHANOSENSITIVE CHANNEL MSCL
  • SUPPORTED LIPID-BILAYERS
  • ION-CHANNEL
  • PROTEIN
  • COLI

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