HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT

KJ MICKLEM; Yin Dong; A WILLIS; KA PULFORD; Lydia Visser; H DURKOP; S POPPEMA; H STEIN; DY MASON

HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT

KJ MICKLEM^*, Yin Dong, A WILLIS, KA PULFORD, Lydia Visser, H DURKOP, S POPPEMA, H STEIN, DY MASON

^*Corresponding author for this work

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

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Abstract

The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160kD, 130kD and 105kD unreduced; 145kD and 120kD reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta-7 cDNA. This molecule is thus a new member of the integrin family of leucoyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.

Original language	English
Pages (from-to)	1297-1301
Number of pages	5
Journal	The American Journal of Pathology
Volume	139
Issue number	6
Publication status	Published - Dec-1991

Keywords

MONOCLONAL-ANTIBODY HML-1
MEMBRANE MOLECULE
LYMPHOCYTES
INTESTINE
SEQUENCE

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@article{811972350a86415da73a0bc532a8bb2c,

title = "HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT",

abstract = "The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160kD, 130kD and 105kD unreduced; 145kD and 120kD reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta-7 cDNA. This molecule is thus a new member of the integrin family of leucoyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.",

keywords = "MONOCLONAL-ANTIBODY HML-1, MEMBRANE MOLECULE, LYMPHOCYTES, INTESTINE, SEQUENCE",

author = "KJ MICKLEM and Yin Dong and A WILLIS and KA PULFORD and Lydia Visser and H DURKOP and S POPPEMA and H STEIN and DY MASON",

year = "1991",

month = dec,

language = "English",

volume = "139",

pages = "1297--1301",

journal = "The American Journal of Pathology",

issn = "0002-9440",

publisher = "ELSEVIER SCIENCE INC",

number = "6",

}

TY - JOUR

T1 - HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT

AU - MICKLEM, KJ

AU - Dong, Yin

AU - WILLIS, A

AU - PULFORD, KA

AU - Visser, Lydia

AU - DURKOP, H

AU - POPPEMA, S

AU - STEIN, H

AU - MASON, DY

PY - 1991/12

Y1 - 1991/12

N2 - The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160kD, 130kD and 105kD unreduced; 145kD and 120kD reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta-7 cDNA. This molecule is thus a new member of the integrin family of leucoyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.

AB - The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160kD, 130kD and 105kD unreduced; 145kD and 120kD reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta-7 cDNA. This molecule is thus a new member of the integrin family of leucoyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.

KW - MONOCLONAL-ANTIBODY HML-1

KW - MEMBRANE MOLECULE

KW - LYMPHOCYTES

KW - INTESTINE

KW - SEQUENCE

M3 - Comment/Letter to the editor

C2 - 1750505

SN - 0002-9440

VL - 139

SP - 1297

EP - 1301

JO - The American Journal of Pathology

JF - The American Journal of Pathology

IS - 6

ER -

HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT

Abstract

Keywords

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