HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT

KJ MICKLEM*, Yin Dong, A WILLIS, KA PULFORD, Lydia Visser, H DURKOP, S POPPEMA, H STEIN, DY MASON

*Corresponding author for this work

    Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

    64 Citations (Scopus)

    Abstract

    The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160kD, 130kD and 105kD unreduced; 145kD and 120kD reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta-7 cDNA. This molecule is thus a new member of the integrin family of leucoyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.

    Original languageEnglish
    Pages (from-to)1297-1301
    Number of pages5
    JournalThe American Journal of Pathology
    Volume139
    Issue number6
    Publication statusPublished - Dec-1991

    Keywords

    • MONOCLONAL-ANTIBODY HML-1
    • MEMBRANE MOLECULE
    • LYMPHOCYTES
    • INTESTINE
    • SEQUENCE

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