Host-device interactions: exposure of lung epithelial cells and fibroblasts to nickel, titanium, or nitinol affect proliferation, reactive oxygen species production, and cellular signaling

Simon D Pouwels*, Alina Sigaeva, Shanna de Boer, Ilse A Eichhorn, Lisanne Koll, Jeroen Kuipers, Romana Schirhagl, Irene H Heijink, Janette K Burgess, Dirk-Jan Slebos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)
28 Downloads (Pure)

Abstract

Endoscopic implantation of medical devices for the treatment of lung diseases, including airway stents, unidirectional valves and coils, is readily used to treat central airway disease and emphysema. However, granulation and fibrotic tissue formation impairs treatment effectiveness. To date little is known about the interaction between implanted devices, often made from metals, such as nickel, titanium or nitinol, and cells in the airways. Here, we study the response of lung epithelial cells and fibroblasts to implant device materials. The adhesion and proliferation of bronchial epithelial cells and lung fibroblasts upon exposure to 10 × 3 × 1 mm pieces of nickel, titanium or nitinol is examined using light and scanning electron microscopy. Pro-inflammatory cytokine mRNA expression and release, signaling kinase activity and intracellular free radical production are assessed. Nitinol, and to a lesser extent nickel and titanium, surfaces support the attachment and growth of lung epithelial cells. Nitinol induces a rapid and significant alteration of kinase activity. Cells directly exposed to nickel or titanium produce free radicals, but those exposed to nitinol do not. The response of lung epithelial cells and fibroblasts depends on the metal type to which they are exposed. Nitinol induces cellular surface growth and the induction of kinase activity, while exposure of lung epithelial cells to nickel and titanium induces free radical production, but nitinol does not.

Original languageEnglish
Pages (from-to)38
Number of pages13
JournalJournal of Materials Science : Materials in Medicine
Volume34
Issue number7
DOIs
Publication statusPublished - 24-Jul-2023

Keywords

  • Nickel
  • Titanium
  • Reactive Oxygen Species
  • Alloys/pharmacology
  • Stents
  • Epithelial Cells
  • Cell Proliferation
  • Fibroblasts
  • Lung

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