Human CD8(+) T cell responses against five newly identified respiratory syncytial virus-derived epitopes

J Heidema, GJ de Bree, PMA de Graaff, WWC van Maren, P Hoogerhout, TA Out, JLL Kimpen, GM van Bleek*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

58 Citations (Scopus)

Abstract

CD8(+) T lymphocytes play a major role in the clearance of respiratory syncytial virus (RSV) infections. To be able to study the primary CTL response in RSV-infected children, epitopes presented by a set of commonly used HLA alleles (HLA-A1, -A3, -B44 and -B51) were searched for. Five epitopes were characterized derived from the matrix (M), non-structural (NS2) and second matrix (M2) proteins of RSV. All epitopes were shown to be processed and presented by RSV-infected antigen-presenting cells. HLA-A1 tetramers for one of these epitopes derived from the M protein were constructed and used to quantify and phenotype the memory CD8(+) T cell pool in a panel of healthy adult donors. In about 60% of the donors, CD8(+) T cells specific for the M protein could be identified. These cells belonged to the memory T cell subset characterized by expression of CD27 and CD28, and down-regulation of CCR7 and CD45RA. The frequency of tetramer-positive cells varied between 0.4 and 3 per 10(4) CD8(+) T cells in PBMC of healthy asymptomatic adult donors.

Original languageEnglish
Pages (from-to)2365-2374
Number of pages10
JournalJournal of general virology
Volume85
DOIs
Publication statusPublished - Aug-2004

Keywords

  • FUSION PROTEIN
  • LYMPHOCYTE EPITOPE
  • CLEAR VIRUS
  • INFECTION
  • BRONCHIOLITIS
  • MEMORY
  • MICE
  • PHENOTYPE
  • CHILDREN
  • DISEASE

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