Abstract
Budding yeast Mps1p kinase has been implicated in both the duplication of microtubule-organizing centers and the spindle assembly checkpoint. Here we show that hMps1, the human homolog of yeast Mps1p, is a cell cycle-regulated kinase with maximal activity during M phase. hMps1 localizes to kinetochores and its activity and phosphorylation state increase upon activation of the mitotic checkpoint. By antibody microinjection and siRNA, we demonstrate that hMps1 is required for human cells to undergo checkpoint arrest in response to microtubule depolymerization. In contrast, centrosome (re-)duplication as well as cell division occur in the absence of hMps1. We conclude that hMps1 is required for the spindle assembly checkpoint but not for centrosome duplication.
| Original language | English |
|---|---|
| Pages (from-to) | 1723-32 |
| Number of pages | 10 |
| Journal | EMBO Journal |
| Volume | 21 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2-Apr-2002 |
| Externally published | Yes |
Keywords
- Animals
- Cell Cycle
- Cell Cycle Proteins
- Centrosome
- Gene Expression
- Gene Silencing
- HeLa Cells
- Humans
- Kinetochores
- Mice
- Mice, Inbred BALB C
- Microinjections
- Mitosis
- Protein Kinases
- Protein-Serine-Threonine Kinases
- Protein-Tyrosine Kinases
- RNA, Small Interfering
- RNA, Untranslated
- Signal Transduction
- Spindle Apparatus
- Tumor Cells, Cultured