Identification of a novel oxygenase capable of regiospecific hydroxylation of D-limonene into (+)-trans-carveol

M. Groeneveld, H. L. van Beek, W. A. Duetz, M. W. Fraaije*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

By genome sequencing and analysis, we have been able to identify a gene cluster encoding a four component oxygenase which is able to oxidize D-limonene, L-limonene, cumene, and indole. Heterologous expression of the complete oxygenase system in Pseudomonas putida S12 allowed efficient and highly regiospecific biotransformation of D-limonene into (+)-trans-carveol. The discovered oxygenase represents a promising biocatalyst for the biotechnological valorisation of D-limonene. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)7263-7267
Number of pages5
JournalTetrahedron
Volume72
Issue number46
DOIs
Publication statusPublished - 17-Nov-2016
Event12th Conference on Modern Developments in Biotransformations (Biotrans) - Vienna, Austria
Duration: 1-Jan-2015 → …

Keywords

  • D-Limone
  • Carveol
  • Carvone
  • Cumene
  • Oxygenase
  • Hydroxylation
  • PSEUDOMONAS-PUTIDA
  • TOLUENE DIOXYGENASE
  • CRYSTAL-STRUCTURE
  • BIOTRANSFORMATION
  • ISOPROPYLBENZENE
  • EXPRESSION
  • PLASMID
  • MONOOXYGENATION
  • METABOLISM
  • COMPONENT

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