Identification of a TLR2 Inhibiting Wheat Hydrolysate

Mensiena B. G. Kiewiet*, Renske Dekkers, Martine P. van Gool, Laurien H. Ulfman, Andre Groeneveld, Marijke M. Faas, Paul de Vos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
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Abstract

Scope Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy-induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll-like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage.

Methods and results The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2-ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL-6 production in human dendritic cells. Application of reversed-phase-ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity.

Conclusion The study demonstrates TLR2-inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy.

Original languageEnglish
Article number1800716
Number of pages10
JournalMolecular Nutrition & Food Research
Volume62
Issue number23
Early online date24-Oct-2018
DOIs
Publication statusPublished - Dec-2018

Keywords

  • bioactive peptides
  • inhibition
  • mucositis
  • toll-like receptor 2
  • wheat hydrolysates
  • DENDRITIC CELLS
  • IMMUNOMODULATING PROPERTIES
  • INTESTINAL MUCOSITIS
  • PROTEIN HYDROLYSATE
  • IN-VITRO
  • RECEPTOR
  • DOXORUBICIN
  • DIETARY
  • GLUTEN
  • INFLAMMATION

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