Identification of asthma associated microRNAs in bronchial biopsies

Mirjam P Roffel, Ilse M Boudewijn, Jos L L van Nijnatten, Alen Faiz, Corneel J Vermeulen, Antoon J van Oosterhout, Karen Affleck, Wim Timens, Ken R Bracke, Tania Maes, Irene H Heijink, Corry-Anke Brandsma, Maarten van den Berge*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)
3 Downloads (Pure)

Abstract

Changes in microRNA (miRNA) expression can contribute to the pathogenesis of many diseases, including asthma. We aimed to identify miRNAs that are differentially expressed between asthma patients and healthy controls and explored their association with clinical and inflammatory parameters of asthma.Differentially expressed miRNAs were determined by small RNA sequencing on bronchial biopsies of 79 asthma patients and 82 healthy controls using linear regression models. Differentially expressed miRNAs were associated with clinical and inflammatory asthma features. Potential miRNA-mRNA interactions were analysed using mRNA data available from the same bronchial biopsies and enrichment of pathways was identified with Enrichr and g:Profiler.In total 78 differentially expressed miRNAs were identified in bronchial biopsies of asthma patients compared to controls, of which 60 remained differentially expressed after controlling for smoke and inhaled corticosteroid treatment. We identified several asthma associated miRNAs, including miR-125b-5p and miR-223-3p, based on a significant association with multiple clinical and inflammatory asthma features and their negative correlation with genes associated with the presence of asthma. The most enriched biological pathway(s) affected by miR-125b-5p and miR-223-3p were inflammatory response and cilium assembly and organisation. Of interest, we identified that lower expression of miR-26a-5p was linked to more severe eosinophilic inflammation as measured in blood, sputum as well as bronchial biopsies. Collectively, we identified miR-125b-5p, miR-223-3p and miR-26a-5p, as potential regulators that could contribute to the pathogenesis of asthma.

Original languageEnglish
Article number2101294
Number of pages12
JournalEuropean Respiratory Journal
Volume59
Issue number3
Early online date26-Aug-2021
DOIs
Publication statusPublished - 17-Mar-2022

Keywords

  • ENRICHMENT ANALYSIS
  • AIRWAY INFLAMMATION
  • WEB SERVER
  • EXPRESSION
  • HEMOGLOBIN
  • EPITHELIUM
  • GREMLIN

Cite this