Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q

Paula Niewold; Douwe J. Dijkstra; Yi Cai; Delia Goletti; Fabrizio Palmieri; Krista E. van Meijgaarden; Frank A.W. Verreck; Onno W. Akkerman; Regina W. Hofland; Eveline M. Delemarre; Stefan Nierkens; Marije K. Verheul; Andrew J. Pollard; Jaap T. van Dissel; Tom H.M. Ottenhoff; Leendert A. Trouw; Simone A. Joosten

doi:10.1038/s41598-023-38889-x

Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q

Paula Niewold^*, Douwe J. Dijkstra, Yi Cai, Delia Goletti, Fabrizio Palmieri, Krista E. van Meijgaarden, Frank A.W. Verreck, Onno W. Akkerman, Regina W. Hofland, Eveline M. Delemarre, Stefan Nierkens, Marije K. Verheul, Andrew J. Pollard, Jaap T. van Dissel, Tom H.M. Ottenhoff, Leendert A. Trouw, Simone A. Joosten

^*Corresponding author for this work

Microbes in Health and Disease (MHD)

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Abstract

Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.

Original language	English
Article number	11617
Number of pages	10
Journal	Scientific Reports
Volume	13
Early online date	18-Jun-2023
DOIs	https://doi.org/10.1038/s41598-023-38889-x
Publication status	Published - 2023

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Identification of circulating monocytes as producers of tuberculosis disease biomarker C1qFinal publisher's version, 1.46 MBLicence: CC BY

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Niewold, P., Dijkstra, D. J., Cai, Y., Goletti, D., Palmieri, F., van Meijgaarden, K. E., Verreck, F. A. W., Akkerman, O. W., Hofland, R. W., Delemarre, E. M., Nierkens, S., Verheul, M. K., Pollard, A. J., van Dissel, J. T., Ottenhoff, T. H. M., Trouw, L. A., & Joosten, S. A. (2023). Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q. Scientific Reports, 13, Article 11617. https://doi.org/10.1038/s41598-023-38889-x

@article{1ff44078f5da4161bc7c2ad8c487543d,

title = "Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q",

abstract = "Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.",

author = "Paula Niewold and Dijkstra, {Douwe J.} and Yi Cai and Delia Goletti and Fabrizio Palmieri and {van Meijgaarden}, {Krista E.} and Verreck, {Frank A.W.} and Akkerman, {Onno W.} and Hofland, {Regina W.} and Delemarre, {Eveline M.} and Stefan Nierkens and Verheul, {Marije K.} and Pollard, {Andrew J.} and {van Dissel}, {Jaap T.} and Ottenhoff, {Tom H.M.} and Trouw, {Leendert A.} and Joosten, {Simone A.}",

note = "Funding Information: This work was partially supported by the following grants: National Institute for Infectious Diseases-IRCCS L. Spallanzani, Rome, Italy; Line 4 four-Ricerca Corrente, funded by the Italian Ministry of Health; European Union EC HORIZON2020 TBVAC2020 (Grant Agreement No. 643381); UK Medical Research Council (MRC) (MR/K021222/1) and the Oxford Biomedical Research Centre. The funders were not involved in the study design, collection, analysis, and interpretation of data, writing of this article, or the decision to submit it for publication. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

doi = "10.1038/s41598-023-38889-x",

language = "English",

volume = "13",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

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Niewold, P, Dijkstra, DJ, Cai, Y, Goletti, D, Palmieri, F, van Meijgaarden, KE, Verreck, FAW, Akkerman, OW, Hofland, RW, Delemarre, EM, Nierkens, S, Verheul, MK, Pollard, AJ, van Dissel, JT, Ottenhoff, THM, Trouw, LA & Joosten, SA 2023, 'Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q', Scientific Reports, vol. 13, 11617. https://doi.org/10.1038/s41598-023-38889-x

TY - JOUR

T1 - Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q

AU - Niewold, Paula

AU - Dijkstra, Douwe J.

AU - Cai, Yi

AU - Goletti, Delia

AU - Palmieri, Fabrizio

AU - van Meijgaarden, Krista E.

AU - Verreck, Frank A.W.

AU - Akkerman, Onno W.

AU - Hofland, Regina W.

AU - Delemarre, Eveline M.

AU - Nierkens, Stefan

AU - Verheul, Marije K.

AU - Pollard, Andrew J.

AU - van Dissel, Jaap T.

AU - Ottenhoff, Tom H.M.

AU - Trouw, Leendert A.

AU - Joosten, Simone A.

N1 - Funding Information: This work was partially supported by the following grants: National Institute for Infectious Diseases-IRCCS L. Spallanzani, Rome, Italy; Line 4 four-Ricerca Corrente, funded by the Italian Ministry of Health; European Union EC HORIZON2020 TBVAC2020 (Grant Agreement No. 643381); UK Medical Research Council (MRC) (MR/K021222/1) and the Oxford Biomedical Research Centre. The funders were not involved in the study design, collection, analysis, and interpretation of data, writing of this article, or the decision to submit it for publication. Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.

AB - Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.

U2 - 10.1038/s41598-023-38889-x

DO - 10.1038/s41598-023-38889-x

M3 - Article

C2 - 37464009

AN - SCOPUS:85165343091

SN - 2045-2322

VL - 13

JO - Scientific Reports

JF - Scientific Reports

M1 - 11617

ER -

Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q

Abstract

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