Identification of sequence motifs responsible for the adhesive interaction between exon v10-containing CD44 isoforms

Gregory M Hayes, Roland Chiu, Carmine Carpenito, Shona T Dougherty, Graeme J Dougherty

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Previous studies have demonstrated that CD44 isoforms containing the alternatively spliced exon v10 promote cell-cell adhesion via a mechanism that involves the recognition of chondroitin sulfate side chains presented on the surface of interacting cells in association with other CD44 molecules. Sequence analysis revealed the presence within exon v10 of two motifs that may be relevant to this interaction, a B[X(7)]B motif that may contribute to the recognition and binding of chondroitin sulfate and a serine-glycine motif that may serve as a site of chondroitin sulfate attachment. To determine whether either of these two motifs explain the unique adhesive activity of exon v10-containing CD44 isoforms, each was targeted by site-directed mutagenesis, and the adhesive activity of the resultant mutants was determined using a quantitative cell-cell binding assay. The data obtained demonstrate conclusively that it is the exon v10-encoded B[X(7)]B motif that is solely responsible for the enhanced adhesive activity of exon v10-containing CD44 isoforms.

Original languageEnglish
Pages (from-to)50529-34
Number of pages6
JournalThe Journal of Biological Chemistry
Volume277
Issue number52
DOIs
Publication statusPublished - 27-Dec-2002

Keywords

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • COS Cells
  • Cell Adhesion
  • Cell Line, Transformed
  • Cercopithecus aethiops
  • Exons
  • Humans
  • Hyaluronan Receptors
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Polymerase Chain Reaction
  • Simian virus 40
  • Journal Article
  • Research Support, U.S. Gov't, P.H.S.

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